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Assay Detail

CHEMBL4055735

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MBP-tagged human CYP24A1 expressed Escherichia coli BL21-Gold(DE3) incubated for 25 mins in presence of Adx, AdR 1,25(OH)2D3 and NADPH by HPLC method
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Synthesis, molecular modelling and CYP24A1 inhibitory activity of novel of (E)-N-(2-(1H-imidazol-1-yl)-2-(phenylethyl)-3/4-styrylbenzamides.
Taban IM, Zhu J, DeLuca HF, Simons C.
Bioorg Med Chem (2017) 25 : 4076 -4087
PubMed 28601511 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 6.70 6.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3329769 1 6.96
CHEMBL4105510 1 6.96
CHEMBL4080128 1 6.92
CHEMBL4066700 1 6.82
CHEMBL4087998 1 6.80
CHEMBL4103700 1 6.75
CHEMBL4104362 1 6.72
CHEMBL4087325 1 6.68
CHEMBL4076840 1 6.51
CHEMBL4060725 1 6.47
CHEMBL4079445 1 6.46
CHEMBL157101 KETOCONAZOLE 4.0 1 6.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3329769 IC50 = 110.0 nM 6.96
CHEMBL4105510 IC50 = 110.0 nM 6.96
CHEMBL4080128 IC50 = 120.0 nM 6.92
CHEMBL4066700 IC50 = 150.0 nM 6.82
CHEMBL4087998 IC50 = 160.0 nM 6.80
CHEMBL4103700 IC50 = 180.0 nM 6.75
CHEMBL4104362 IC50 = 190.0 nM 6.72
CHEMBL4087325 IC50 = 210.0 nM 6.68
CHEMBL4076840 IC50 = 310.0 nM 6.51
CHEMBL4060725 IC50 = 340.0 nM 6.47
CHEMBL4079445 IC50 = 350.0 nM 6.46
CHEMBL157101 KETOCONAZOLE IC50 = 500.0 nM 6.30