Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4056672

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human G9a catalytic domain (913 to 1193 residues) expressed in Escherichia coli BL21 (DE3) using biotinylated H3 (1 to 25 residues) as substrate preincubated for 20 mins in presence of [3H]SAM measured after 2 hrs by scintillation proximity assay
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone-lysine N-methyltransferase EHMT2 (CHEMBL6032)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-activity relationship studies of G9a-like protein (GLP) inhibitors.
Xiong Y, Li F, Babault N, Wu H, Dong A, Zeng H, Chen X, Arrowsmith CH, Brown PJ, Liu J, Vedadi M, Jin J.
Bioorg Med Chem (2017) 25 : 4414 -4423
PubMed 28662962 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 6.38 7.04

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4066769 1 7.04
CHEMBL4088064 1 6.96
CHEMBL4075387 1 6.82
CHEMBL4100004 1 6.75
CHEMBL576260 1 6.72
CHEMBL4094717 1 6.59
CHEMBL4069640 1 6.55
CHEMBL4072211 1 6.53
CHEMBL4084508 1 6.50
CHEMBL4104425 1 6.50
CHEMBL585180 1 6.46
CHEMBL4074209 1 6.45
CHEMBL4064559 1 6.45
CHEMBL4101782 1 6.35
CHEMBL4092975 1 6.33
CHEMBL4063767 1 6.32
CHEMBL4072358 1 6.32
CHEMBL4105129 1 6.29
CHEMBL4090654 1 6.27
CHEMBL4068291 1 6.27
CHEMBL4102467 1 6.26
CHEMBL4093972 1 6.23
CHEMBL4095652 1 6.14
CHEMBL4092251 1 6.14
CHEMBL4080189 1 6.10
CHEMBL4083783 1 6.02
CHEMBL4073147 1 5.94
CHEMBL4076634 1 5.80
CHEMBL4082847 1 5.79

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4066769 IC50 = 92.0 nM 7.04
CHEMBL4088064 IC50 = 110.0 nM 6.96
CHEMBL4075387 IC50 = 151.0 nM 6.82
CHEMBL4100004 IC50 = 177.0 nM 6.75
CHEMBL576260 IC50 = 191.0 nM 6.72
CHEMBL4094717 IC50 = 259.0 nM 6.59
CHEMBL4069640 IC50 = 282.0 nM 6.55
CHEMBL4072211 IC50 = 295.0 nM 6.53
CHEMBL4084508 IC50 = 318.0 nM 6.50
CHEMBL4104425 IC50 = 315.0 nM 6.50
CHEMBL585180 IC50 = 343.0 nM 6.46
CHEMBL4074209 IC50 = 356.0 nM 6.45
CHEMBL4064559 IC50 = 359.0 nM 6.45
CHEMBL4101782 IC50 = 443.0 nM 6.35
CHEMBL4092975 IC50 = 465.0 nM 6.33
CHEMBL4063767 IC50 = 481.0 nM 6.32
CHEMBL4072358 IC50 = 484.0 nM 6.32
CHEMBL4105129 IC50 = 508.0 nM 6.29
CHEMBL4090654 IC50 = 534.0 nM 6.27
CHEMBL4068291 IC50 = 532.0 nM 6.27
CHEMBL4102467 IC50 = 550.0 nM 6.26
CHEMBL4093972 IC50 = 585.0 nM 6.23
CHEMBL4095652 IC50 = 726.0 nM 6.14
CHEMBL4092251 IC50 = 724.0 nM 6.14
CHEMBL4080189 IC50 = 788.0 nM 6.10
CHEMBL4083783 IC50 = 956.0 nM 6.02
CHEMBL4073147 IC50 = 1160.0 nM 5.94
CHEMBL4076634 IC50 = 1582.0 nM 5.80
CHEMBL4082847 IC50 = 1630.0 nM 5.79