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Assay Detail

CHEMBL4059304

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]cytisine from human alpha4beta2 nAChR expressed in human SH-EP1 cell membranes after 75 mins by liquid scintillation spectrometry
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907589)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Dual Nicotinic Acetylcholine Receptor α4β2 Antagonists/α7 Agonists: Synthesis, Docking Studies, and Pharmacological Evaluation of Tetrahydroisoquinolines and Tetrahydroisoquinolinium Salts.
Crestey F, Jensen AA, Soerensen C, Magnus CB, Andreasen JT, Peters GHJ, Kristensen JL.
J Med Chem (2018) 61 : 1719 -1729
PubMed 29384668 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 2 6.76 7.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL446533 ERYSODINE 1 7.30
CHEMBL442947 ERYSOTRINE 1 6.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL446533 ERYSODINE Ki = 50.0 nM 7.30
CHEMBL442947 ERYSOTRINE Ki = 600.0 nM 6.22