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Assay Detail

CHEMBL4059396

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human N-terminal MBP-fused NEU2 expressed in Escherichia coli using 4MU-NANA as substrate preincubated for 15 mins followed by substrate addition measured every minute for 30 mins by fluorescence based assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sialidase-2 (CHEMBL3200)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Selective Inhibitors of Human Neuraminidase 3.
Guo T, Dätwyler P, Demina E, Richards MR, Ge P, Zou C, Zheng R, Fougerat A, Pshezhetsky AV, Ernst B, Cairo CW.
J Med Chem (2018) 61 : 1990 -2008
PubMed 29425031 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 4.73 5.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL222813 ZANAMIVIR 4.0 1 5.24
CHEMBL4099818 1 4.77
CHEMBL96712 NEU5AC2EN 1 4.60
CHEMBL4076203 1 4.32

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL222813 ZANAMIVIR Ki = 5700.0 nM 5.24
CHEMBL4099818 Ki = 17000.0 nM 4.77
CHEMBL96712 NEU5AC2EN Ki = 25000.0 nM 4.60
CHEMBL4076203 Ki = 48000.0 nM 4.32