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Assay Detail

CHEMBL4119773

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant 6His-tagged USP7 using Ub-Rh110 as substrate preincubated for 30 mins followed by substrate addition measured after 180 mins by fluorometric analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Ubiquitin carboxyl-terminal hydrolase 7 (CHEMBL2157850)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Chemical Approaches to Intervening in Ubiquitin Specific Protease 7 (USP7) Function for Oncology and Immune Oncology Therapies.
Wu J, Kumar S, Wang F, Wang H, Chen L, Arsenault P, Mattern M, Weinstock J.
J Med Chem (2018) 61 : 422 -443
PubMed 28768102 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 6.44 6.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL510492 XESTOQUINONE 1 6.89
CHEMBL4128416 1 6.31
CHEMBL4126079 1 6.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL510492 XESTOQUINONE IC50 = 130.0 nM 6.89
CHEMBL4128416 IC50 = 490.0 nM 6.31
CHEMBL4126079 IC50 = 750.0 nM 6.12