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Assay Detail

CHEMBL4121164

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MERS-CoV spike protein Q1020R mutant mediated cell-cell fusion between viral protein expressing human 293T cells and human HuH7 target cells assessed as decrease in pseudovirus infection preincubated with virus for 1 hr followed by HuH7 cells addition measured after 48 hrs by luciferase reporter gene assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Middle East respiratory syndrome-related coronavirus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of Hydrocarbon-Stapled Short α-Helical Peptides as Promising Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Fusion Inhibitors.
Wang C, Xia S, Zhang P, Zhang T, Wang W, Tian Y, Meng G, Jiang S, Liu K.
J Med Chem (2018) 61 : 2018 -2026
PubMed 29442512 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 4 5.89 6.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4127413 1 6.19
CHEMBL4126311 1 6.05
CHEMBL4130273 1 5.70
CHEMBL4127619 1 5.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4127413 EC50 = 640.0 nM 6.19
CHEMBL4126311 EC50 = 890.0 nM 6.05
CHEMBL4130273 EC50 = 1980.0 nM 5.70
CHEMBL4127619 EC50 = 2490.0 nM 5.60