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Assay Detail

CHEMBL4122270

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of doxycycline induced Hepsin in human MCF10A cells using Boc-QRR-AMC as substrate preincubated for 24 hrs followed by doxycycline induction for 24 hrs and subsequent addition of substrate by fluorescence assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine protease hepsin (CHEMBL2079849)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, Synthesis, and Testing of Potent, Selective Hepsin Inhibitors via Application of an Automated Closed-Loop Optimization Platform.
Pant SM, Mukonoweshuro A, Desai B, Ramjee MK, Selway CN, Tarver GJ, Wright AG, Birchall K, Chapman TM, Tervonen TA, Klefström J.
J Med Chem (2018) 61 : 4335 -4347
PubMed 29701962 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 5.92 7.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4129836 1 7.00
CHEMBL4130135 1 6.00
CHEMBL4128554 1 5.89
CHEMBL4127452 1 5.50
CHEMBL4129630 1 5.19

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4129836 IC50 = 100.0 nM 7.00
CHEMBL4130135 IC50 = 990.0 nM 6.00
CHEMBL4128554 IC50 = 1290.0 nM 5.89
CHEMBL4127452 IC50 = 3150.0 nM 5.50
CHEMBL4129630 IC50 = 6490.0 nM 5.19