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Assay Detail

CHEMBL4122799

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length recombinant His6-tagged human PARP14 WWA/catalytic domain expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by biotinylated NAD+ addition by chemiluminescence assay
30
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein mono-ADP-ribosyltransferase PARP14 (CHEMBL2176777)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and evaluation of potent and selective inhibitors of mono-(ADP-ribosyl)transferases PARP10 and PARP14.
Holechek J, Lease R, Thorsell AG, Karlberg T, McCadden C, Grant R, Keen A, Callahan E, Schüler H, Ferraris D.
Bioorg Med Chem Lett (2018) 28 : 2050 -2054
PubMed 29748053 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 5.59 6.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4126260 2 6.16
CHEMBL4129001 2 6.11
CHEMBL4126241 2 5.98
CHEMBL4127497 2 5.97
CHEMBL4125748 2 5.89
CHEMBL4128574 2 5.84
CHEMBL4127327 2 5.62
CHEMBL4129542 2 5.50
CHEMBL4128256 2 5.44
CHEMBL4127476 2 5.38
CHEMBL4129782 2 5.33
CHEMBL4130035 2 5.32
CHEMBL4129789 2 5.28
CHEMBL4126166 2 5.24
CHEMBL1438938 2 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4126260 IC50 = 700.0 nM 6.16
CHEMBL4126260 IC50 = 691.83 nM 6.16
CHEMBL4129001 IC50 = 776.25 nM 6.11
CHEMBL4129001 IC50 = 780.0 nM 6.11
CHEMBL4126241 IC50 = 1047.13 nM 5.98
CHEMBL4127497 IC50 = 1071.52 nM 5.97
CHEMBL4125748 IC50 = 1288.25 nM 5.89
CHEMBL4125748 IC50 = 1300.0 nM 5.89
CHEMBL4126241 IC50 = 1300.0 nM 5.89
CHEMBL4128574 IC50 = 1445.44 nM 5.84
CHEMBL4128574 IC50 = 1500.0 nM 5.82
CHEMBL4127497 IC50 = 1900.0 nM 5.72
CHEMBL4127327 IC50 = 2398.83 nM 5.62
CHEMBL4127327 IC50 = 2430.0 nM 5.61
CHEMBL4129542 IC50 = 3200.0 nM 5.50
CHEMBL4129542 IC50 = 3235.94 nM 5.49
CHEMBL4128256 IC50 = 3630.78 nM 5.44
CHEMBL4128256 IC50 = 3600.0 nM 5.44
CHEMBL4127476 IC50 = 4200.0 nM 5.38
CHEMBL4127476 IC50 = 4265.8 nM 5.37
CHEMBL4129782 IC50 = 4700.0 nM 5.33
CHEMBL4129782 IC50 = 4677.35 nM 5.33
CHEMBL4130035 IC50 = 4800.0 nM 5.32
CHEMBL4130035 IC50 = 4786.3 nM 5.32
CHEMBL4129789 IC50 = 5200.0 nM 5.28
CHEMBL4129789 IC50 = 5248.07 nM 5.28
CHEMBL4126166 IC50 = 5800.0 nM 5.24
CHEMBL4126166 IC50 = 5754.4 nM 5.24
CHEMBL1438938 IC50 = 10000.0 nM 5.00
CHEMBL1438938 IC50 = 10000.0 nM 5.00