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Assay Detail

CHEMBL4122824

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human TRPC6 expressed in HEK293 cells assessed as decrease in intracellular calcium level after 24 hrs by Fluo-4 dye-based FLIPR assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Short transient receptor potential channel 6 (CHEMBL2417347)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a bicyclo[4.3.0]nonane derivative DS88790512 as a potent, selective, and orally bioavailable blocker of transient receptor potential canonical 6 (TRPC6).
Motoyama K, Nagata T, Kobayashi J, Nakamura A, Miyoshi N, Kazui M, Sakurai K, Sakakura T.
Bioorg Med Chem Lett (2018) 28 : 2222 -2227
PubMed 29752182 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 7.13 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4129456 1 8.52
CHEMBL4129805 1 8.10
CHEMBL4129809 1 8.02
CHEMBL4127600 1 7.96
CHEMBL4126041 1 7.66
CHEMBL4128079 1 7.52
CHEMBL4128782 1 7.48
CHEMBL4127190 1 7.43
CHEMBL4125756 1 7.31
CHEMBL4126989 1 7.28
CHEMBL4130214 1 6.80
CHEMBL4129002 1 6.70
CHEMBL4126938 1 6.68
CHEMBL4126435 1 6.55
CHEMBL4126528 1 6.41
CHEMBL4127992 1 6.36
CHEMBL4129133 1 6.30
CHEMBL4129913 1 6.25
CHEMBL4125839 1 6.18

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4129456 IC50 = 3.0 nM 8.52
CHEMBL4129805 IC50 = 8.0 nM 8.10
CHEMBL4129809 IC50 = 9.5 nM 8.02
CHEMBL4127600 IC50 = 11.0 nM 7.96
CHEMBL4126041 IC50 = 22.0 nM 7.66
CHEMBL4128079 IC50 = 30.0 nM 7.52
CHEMBL4128782 IC50 = 33.0 nM 7.48
CHEMBL4127190 IC50 = 37.0 nM 7.43
CHEMBL4125756 IC50 = 49.0 nM 7.31
CHEMBL4126989 IC50 = 52.0 nM 7.28
CHEMBL4130214 IC50 = 160.0 nM 6.80
CHEMBL4129002 IC50 = 200.0 nM 6.70
CHEMBL4126938 IC50 = 210.0 nM 6.68
CHEMBL4126435 IC50 = 280.0 nM 6.55
CHEMBL4126528 IC50 = 390.0 nM 6.41
CHEMBL4127992 IC50 = 440.0 nM 6.36
CHEMBL4129133 IC50 = 500.0 nM 6.30
CHEMBL4129913 IC50 = 560.0 nM 6.25
CHEMBL4125839 IC50 = 660.0 nM 6.18