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Assay Detail

CHEMBL4122979

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of C-terminally biotinylated-H3K4me3 (1 to 21 residues) peptide from KDM7A (PHD-JmjC) (unknown origin) preincubated for 15 mins followed by peptide addition measured after 1 hr by luminescence-based AlphaScreen assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysine-specific demethylase 7A (CHEMBL2163177)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Investigations on small molecule inhibitors targeting the histone H3K4 tri-methyllysine binding PHD-finger of JmjC histone demethylases.
Bhushan B, Erdmann A, Zhang Y, Belle R, Johannson C, Oppermann U, Hopkinson RJ, Schofield CJ, Kawamura A.
Bioorg Med Chem (2018) 26 : 2984 -2991
PubMed 29764755 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 4.55 5.25

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4126406 1 5.25
CHEMBL4129540 1 4.80
CHEMBL633 AMIODARONE 4.0 1 4.10
CHEMBL4128152 1 4.06
CHEMBL4126951 1 -
CHEMBL4130224 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4126406 IC50 = 5600.0 nM 5.25
CHEMBL4129540 IC50 = 16000.0 nM 4.80
CHEMBL633 AMIODARONE IC50 = 80000.0 nM 4.10
CHEMBL4128152 IC50 = 88000.0 nM 4.06
CHEMBL4126951 IC50 = 295000.0 nM -
CHEMBL4130224 IC50 = 149000.0 nM -