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Assay Detail

CHEMBL4123713

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Norovirus prototypic GI.1 3CL protease using HiLyte Fluor 488-labelled DFELQGPK as substrate incubated for 90 mins measured every mins for 20 mins by FRET assay
26
Total Activities
13
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Norovirus GI.1
Confidence 8 — Homologous single protein target
Curated By Intermediate

Target

Nonstructural polyprotein (CHEMBL4295553)
Type SINGLE PROTEIN
Organism Norovirus Hu/GI/10360/2010/VNM

Publication

Synthesis and antiviral evaluation of novel peptidomimetics as norovirus protease inhibitors.
Amblard F, Zhou S, Liu P, Yoon J, Cox B, Muzzarelli K, Kuiper BD, Kovari LC, Schinazi RF.
Bioorg Med Chem Lett (2018) 28 : 2165 -2170
PubMed 29779977 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 6.64 7.50
IC90 13 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2441741 2 7.50
CHEMBL4129318 2 7.10
CHEMBL4128616 2 6.23
CHEMBL4128223 2 6.18
CHEMBL4127236 2 6.18
CHEMBL4128485 2 -
CHEMBL4125865 2 -
CHEMBL4127247 2 -
CHEMBL4126005 2 -
CHEMBL4128073 2 -
CHEMBL4129451 2 -
CHEMBL4126846 2 -
CHEMBL4127374 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2441741 IC50 = 32.0 nM 7.50
CHEMBL4129318 IC50 = 80.0 nM 7.10
CHEMBL4128616 IC50 = 593.0 nM 6.23
CHEMBL4128223 IC50 = 654.0 nM 6.18
CHEMBL4127236 IC50 = 654.0 nM 6.18
CHEMBL4128485 IC50 > 1000.0 nM -
CHEMBL4125865 IC50 > 100000.0 nM -
CHEMBL4127247 IC50 > 1000.0 nM -
CHEMBL4126005 IC50 > 10000.0 nM -
CHEMBL4128073 IC50 > 10000.0 nM -
CHEMBL4129451 IC50 > 10000.0 nM -
CHEMBL4126846 IC50 > 100000.0 nM -
CHEMBL4127374 IC50 > 100000.0 nM -
CHEMBL4128485 IC90 - -
CHEMBL4125865 IC90 - -
CHEMBL4127247 IC90 - -
CHEMBL4129318 IC90 = 155.0 nM -
CHEMBL4126005 IC90 - -
CHEMBL4128073 IC90 - -
CHEMBL4129451 IC90 - -
CHEMBL4126846 IC90 - -
CHEMBL4128223 IC90 = 2241.0 nM -
CHEMBL4128616 IC90 = 1697.0 nM -
CHEMBL4127236 IC90 = 2241.0 nM -
CHEMBL4127374 IC90 - -
CHEMBL2441741 IC90 = 248.0 nM -