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Assay Detail

CHEMBL4123722

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human 20S constitutive proteasome beta-5 using Suc-LLVY-AMC as substrate after 60 mins by fluorescence assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proteasome Macropain subunit MB1 (CHEMBL4662)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Potent and Highly Selective Inhibitors of the Proteasome Trypsin-like Site by Incorporation of Basic Side Chain Containing Amino Acid Derived Sulfonyl Fluorides.
Artschwager R, Ward DJ, Gannon S, Brouwer AJ, van de Langemheen H, Kowalski H, Liskamp RMJ.
J Med Chem (2018) 61 : 5395 -5411
PubMed 29782167 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 5.05 6.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4129042 1 6.68
CHEMBL4128898 1 6.02
CHEMBL2326533 1 5.58
CHEMBL4125911 1 5.17
CHEMBL4128478 1 5.05
CHEMBL4129220 1 5.00
CHEMBL4126411 1 4.75
CHEMBL4129319 1 4.56
CHEMBL4126693 1 4.41
CHEMBL4130258 1 4.17
CHEMBL4127928 1 4.16
CHEMBL4128074 1 -
CHEMBL4127967 1 -
CHEMBL4130108 1 -
CHEMBL4129702 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4129042 IC50 = 210.0 nM 6.68
CHEMBL4128898 IC50 = 950.0 nM 6.02
CHEMBL2326533 IC50 = 2600.0 nM 5.58
CHEMBL4125911 IC50 = 6700.0 nM 5.17
CHEMBL4128478 IC50 = 8900.0 nM 5.05
CHEMBL4129220 IC50 = 10000.0 nM 5.00
CHEMBL4126411 IC50 = 18000.0 nM 4.75
CHEMBL4129319 IC50 = 27800.0 nM 4.56
CHEMBL4126693 IC50 = 39000.0 nM 4.41
CHEMBL4130258 IC50 = 67000.0 nM 4.17
CHEMBL4127928 IC50 = 69000.0 nM 4.16
CHEMBL4128074 IC50 = 140000.0 nM -
CHEMBL4127967 IC50 > 100000.0 nM -
CHEMBL4130108 IC50 > 100000.0 nM -
CHEMBL4129702 IC50 = 125000.0 nM -