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Assay Detail

CHEMBL4124181

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human JAK1 using poly[Glu:Tyr] (4:1) as substrate in presence of 10 uM [gamma33P]ATP by radiometric method
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK1 (CHEMBL2835)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development, Optimization, and Structure-Activity Relationships of Covalent-Reversible JAK3 Inhibitors Based on a Tricyclic Imidazo[5,4- d]pyrrolo[2,3- b]pyridine Scaffold.
Forster M, Chaikuad A, Dimitrov T, Döring E, Holstein J, Berger BT, Gehringer M, Ghoreschi K, Müller S, Knapp S, Laufer SA.
J Med Chem (2018) 61 : 5350 -5366
PubMed 29852068 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 7.68 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL221959 TOFACITINIB 4.0 1 9.30
CHEMBL4125719 1 8.40
CHEMBL4128266 1 7.92
CHEMBL4127590 1 7.80
CHEMBL4128410 1 7.72
CHEMBL4126514 1 7.35
CHEMBL4128181 1 7.28
CHEMBL4125861 1 6.93
CHEMBL1650951 1 6.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL221959 TOFACITINIB IC50 = 0.5 nM 9.30
CHEMBL4125719 IC50 = 4.0 nM 8.40
CHEMBL4128266 IC50 = 12.0 nM 7.92
CHEMBL4127590 IC50 = 16.0 nM 7.80
CHEMBL4128410 IC50 = 19.0 nM 7.72
CHEMBL4126514 IC50 = 45.0 nM 7.35
CHEMBL4128181 IC50 = 52.0 nM 7.28
CHEMBL4125861 IC50 = 117.0 nM 6.93
CHEMBL1650951 IC50 = 344.0 nM 6.46