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Assay Detail

CHEMBL4124785

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human CYP11B2 expressed in Chinese hamster V79 cell mitochondria assessed as reduction in aldosterone production using deoxycorticosterone as substrate after 1.5 hrs in presence of NADPH by HTRF assay
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type V79
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 11B2, mitochondrial (CHEMBL2722)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Novel Pyrazole-Based Selective Aldosterone Synthase (CYP11B2) Inhibitors: A New Template to Coordinate the Heme-Iron Motif of CYP11B2.
Sakakibara R, Sasaki W, Onda Y, Yamaguchi M, Ushirogochi H, Hiraga Y, Sato K, Nishio M, Egi Y, Takedomi K, Shimizu H, Ohbora T, Akahoshi F.
J Med Chem (2018) 61 : 5594 -5608
PubMed 29878770 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 28 7.94 8.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3099695 OSILODROSTAT 4.0 1 8.92
CHEMBL4127675 1 8.66
CHEMBL4126893 1 8.66
CHEMBL4127904 1 8.64
CHEMBL4125850 1 8.60
CHEMBL4129296 1 8.49
CHEMBL4128315 1 8.43
CHEMBL4129177 1 8.40
CHEMBL4125736 1 8.38
CHEMBL4129619 1 8.33
CHEMBL4128167 1 8.27
CHEMBL4127082 1 8.17
CHEMBL4129549 1 8.04
CHEMBL4129146 1 7.92
CHEMBL4126118 1 7.89
CHEMBL4127939 1 7.85
CHEMBL4125707 1 7.58
CHEMBL4128736 1 7.54
CHEMBL4126932 1 7.47
CHEMBL4129577 1 7.15
CHEMBL4126275 1 7.14
CHEMBL4128486 1 6.82
CHEMBL4128329 1 6.55
CHEMBL4127095 1 6.54
CHEMBL4128829 1 -
CHEMBL4129567 1 -
CHEMBL4129712 1 -
CHEMBL4127248 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3099695 OSILODROSTAT IC50 = 1.2 nM 8.92
CHEMBL4127675 IC50 = 2.2 nM 8.66
CHEMBL4126893 IC50 = 2.2 nM 8.66
CHEMBL4127904 IC50 = 2.3 nM 8.64
CHEMBL4125850 IC50 = 2.5 nM 8.60
CHEMBL4129296 IC50 = 3.2 nM 8.49
CHEMBL4128315 IC50 = 3.7 nM 8.43
CHEMBL4129177 IC50 = 4.0 nM 8.40
CHEMBL4125736 IC50 = 4.2 nM 8.38
CHEMBL4129619 IC50 = 4.7 nM 8.33
CHEMBL4128167 IC50 = 5.4 nM 8.27
CHEMBL4127082 IC50 = 6.7 nM 8.17
CHEMBL4129549 IC50 = 9.2 nM 8.04
CHEMBL4129146 IC50 = 12.0 nM 7.92
CHEMBL4126118 IC50 = 13.0 nM 7.89
CHEMBL4127939 IC50 = 14.0 nM 7.85
CHEMBL4125707 IC50 = 26.0 nM 7.58
CHEMBL4128736 IC50 = 29.0 nM 7.54
CHEMBL4126932 IC50 = 34.0 nM 7.47
CHEMBL4129577 IC50 = 71.0 nM 7.15
CHEMBL4126275 IC50 = 72.0 nM 7.14
CHEMBL4128486 IC50 = 150.0 nM 6.82
CHEMBL4128329 IC50 = 280.0 nM 6.55
CHEMBL4127095 IC50 = 290.0 nM 6.54
CHEMBL4128829 IC50 > 1000.0 nM -
CHEMBL4129567 IC50 > 1000.0 nM -
CHEMBL4129712 IC50 > 1000.0 nM -
CHEMBL4127248 IC50 > 1000.0 nM -