Assay Detail
Binding
CHEMBL4132608
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human N-terminal His6-MBP-tagged PSA expressed in Escherichia coli BL21 STAR (DE3) using 4-Ala-MNA as substrate measured for 30 mins by thefluorescence assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Puromycin based inhibitors of aminopeptidases for the potential treatment of hematologic malignancies.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 11 | 5.62 | 8.13 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3244800 | — | — | 1 | 8.13 |
| CHEMBL2103847 | TOSEDOSTAT | 2.0 | 1 | 6.58 |
| CHEMBL3244799 | — | — | 1 | 6.03 |
| CHEMBL4165328 | — | — | 1 | 5.85 |
| CHEMBL29292 | UBENIMEX | 2.0 | 1 | 5.46 |
| CHEMBL4161892 | — | — | 1 | 5.32 |
| CHEMBL4161496 | — | — | 1 | 5.30 |
| CHEMBL469912 | PUROMYCIN | 2.0 | 1 | 5.01 |
| CHEMBL4173240 | — | — | 1 | 4.28 |
| CHEMBL4161744 | — | — | 1 | 4.28 |
| CHEMBL4169814 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3244800 | — | IC50 | = | 7.4 | nM | 8.13 |
| CHEMBL2103847 | TOSEDOSTAT | IC50 | = | 260.0 | nM | 6.58 |
| CHEMBL3244799 | — | IC50 | = | 940.0 | nM | 6.03 |
| CHEMBL4165328 | — | IC50 | = | 1400.0 | nM | 5.85 |
| CHEMBL29292 | UBENIMEX | IC50 | = | 3500.0 | nM | 5.46 |
| CHEMBL4161892 | — | IC50 | = | 4800.0 | nM | 5.32 |
| CHEMBL4161496 | — | IC50 | = | 5000.0 | nM | 5.30 |
| CHEMBL469912 | PUROMYCIN | IC50 | = | 9700.0 | nM | 5.01 |
| CHEMBL4173240 | — | IC50 | = | 53000.0 | nM | 4.28 |
| CHEMBL4161744 | — | IC50 | = | 53000.0 | nM | 4.28 |
| CHEMBL4169814 | — | IC50 | > | 100000.0 | nM | - |