Assay Detail
Binding
CHEMBL4132673
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant mouse N-terminal FLAG-tagged Notch1 (1704 to 2531 residues) expressed in human LS174T cells assessed as reduction in doxycycline-induced NICD expression after 12 hrs by Bright-Glo luciferase reporter gene assay
16
Total Activities
8
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | LS174T |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Neurogenic locus notch homolog protein 1 (CHEMBL2146346) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Notch Inhibitors from Calotropis gigantea That Induce Neuronal Differentiation of Neural Stem Cells.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 5.97 | 6.52 |
| Inhibition | 8 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4164399 | — | — | 2 | 6.52 |
| CHEMBL445086 | ASCLEPIN | — | 2 | 6.52 |
| CHEMBL500585 | USCHARIN | — | 2 | 6.40 |
| CHEMBL4166735 | — | — | 2 | 6.16 |
| CHEMBL495949 | CALOTROPIN | — | 2 | 6.10 |
| CHEMBL360322 | — | — | 2 | 5.70 |
| CHEMBL449207 | USCHARIDIN | — | 2 | 5.62 |
| CHEMBL4176111 | — | — | 2 | 4.76 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4164399 | — | IC50 | = | 300.0 | nM | 6.52 |
| CHEMBL445086 | ASCLEPIN | IC50 | = | 300.0 | nM | 6.52 |
| CHEMBL500585 | USCHARIN | IC50 | = | 400.0 | nM | 6.40 |
| CHEMBL4166735 | — | IC50 | = | 700.0 | nM | 6.16 |
| CHEMBL495949 | CALOTROPIN | IC50 | = | 800.0 | nM | 6.10 |
| CHEMBL360322 | — | IC50 | = | 2000.0 | nM | 5.70 |
| CHEMBL449207 | USCHARIDIN | IC50 | = | 2400.0 | nM | 5.62 |
| CHEMBL4176111 | — | IC50 | = | 17300.0 | nM | 4.76 |
| CHEMBL500585 | USCHARIN | Inhibition | - | % | - | |
| CHEMBL445086 | ASCLEPIN | Inhibition | - | % | - | |
| CHEMBL4164399 | — | Inhibition | - | % | - | |
| CHEMBL495949 | CALOTROPIN | Inhibition | - | % | - | |
| CHEMBL360322 | — | Inhibition | - | % | - | |
| CHEMBL449207 | USCHARIDIN | Inhibition | - | % | - | |
| CHEMBL4176111 | — | Inhibition | - | % | - | |
| CHEMBL4166735 | — | Inhibition | - | % | - |