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Assay Detail

CHEMBL4132698

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of 11beta-HSD1 in human liver microsomes using [3H]cortisone as substrate preincubated for 15 mins followed by substrate addition and measured after 30 mins by scintillation proximity assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Liver
Subcellular microsomes
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

11-beta-hydroxysteroid dehydrogenase 1 (CHEMBL4235)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and in vivo study of novel pyrrolidine-based 11β-HSD1 inhibitors for age-related cognitive dysfunction.
Leiva R, Griñan-Ferré C, Seira C, Valverde E, McBride A, Binnie M, Pérez B, Luque FJ, Pallàs M, Bidon-Chanal A, Webster SP, Vázquez S.
Eur J Med Chem (2017) 139 : 412 -428
PubMed 28818766 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 6.85 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4170932 1 7.70
CHEMBL4163698 1 7.70
CHEMBL4170544 1 7.70
CHEMBL4162610 1 7.52
CHEMBL4160328 1 7.52
CHEMBL4168636 1 7.40
CHEMBL4174155 1 7.30
CHEMBL1095047 1 7.05
CHEMBL4174368 1 7.05
CHEMBL4172177 1 6.54
CHEMBL4160886 1 6.50
CHEMBL4166243 1 6.39
CHEMBL4164290 1 5.97
CHEMBL4160714 1 5.91
CHEMBL4167147 1 5.83
CHEMBL4168826 1 5.56
CHEMBL4159148 1 -
CHEMBL4169794 1 -
CHEMBL4159236 1 -
CHEMBL4175606 1 -
CHEMBL4167737 1 -
CHEMBL4160506 1 -
CHEMBL4171112 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4170932 IC50 = 20.0 nM 7.70
CHEMBL4163698 IC50 = 20.0 nM 7.70
CHEMBL4170544 IC50 = 20.0 nM 7.70
CHEMBL4162610 IC50 = 30.0 nM 7.52
CHEMBL4160328 IC50 = 30.0 nM 7.52
CHEMBL4168636 IC50 = 40.0 nM 7.40
CHEMBL4174155 IC50 = 50.0 nM 7.30
CHEMBL1095047 IC50 = 90.0 nM 7.05
CHEMBL4174368 IC50 = 90.0 nM 7.05
CHEMBL4172177 IC50 = 290.0 nM 6.54
CHEMBL4160886 IC50 = 320.0 nM 6.50
CHEMBL4166243 IC50 = 410.0 nM 6.39
CHEMBL4164290 IC50 = 1080.0 nM 5.97
CHEMBL4160714 IC50 = 1220.0 nM 5.91
CHEMBL4167147 IC50 = 1490.0 nM 5.83
CHEMBL4168826 IC50 = 2770.0 nM 5.56
CHEMBL4159148 IC50 - -
CHEMBL4169794 IC50 - -
CHEMBL4159236 IC50 - -
CHEMBL4175606 IC50 - -
CHEMBL4167737 IC50 - -
CHEMBL4160506 IC50 - -
CHEMBL4171112 IC50 - -