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Assay Detail

CHEMBL4133149

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at P2X7 receptor in Swiss mouse peritoneal macrophages assessed as inhibition of ATP-induced current at holding potential of -60 mV preincubated for 5 mins followed by ATP addition measured after 5 mins by Whole cell patch-clamp analysis
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2X purinoceptor 7 (CHEMBL5183)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

1-Aryl-1H- and 2-aryl-2H-1,2,3-triazole derivatives blockade P2X7 receptor in vitro and inflammatory response in vivo.
Gonzaga DTG, Ferreira LBG, Moreira Maramaldo Costa TE, von Ranke NL, Anastácio Furtado Pacheco P, Sposito Simões AP, Arruda JC, Dantas LP, de Freitas HR, de Melo Reis RA, Penido C, Bello ML, Castro HC, Rodrigues CR, Ferreira VF, Faria RX, da Silva FC.
Eur J Med Chem (2017) 139 : 698 -717
PubMed 28858765 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 6.81 7.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4162056 1 7.16
CHEMBL4176461 1 7.09
CHEMBL4161422 1 6.99
CHEMBL1823297 1 6.99
CHEMBL4173394 BRILLIANT BLUE G 4.0 1 6.96
CHEMBL4166558 1 6.50
CHEMBL1823287 1 6.47
CHEMBL4161877 1 6.34

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4162056 IC50 = 69.0 nM 7.16
CHEMBL4176461 IC50 = 81.0 nM 7.09
CHEMBL4161422 IC50 = 103.0 nM 6.99
CHEMBL1823297 IC50 = 103.0 nM 6.99
CHEMBL4173394 BRILLIANT BLUE G IC50 = 109.0 nM 6.96
CHEMBL4166558 IC50 = 316.0 nM 6.50
CHEMBL1823287 IC50 = 341.0 nM 6.47
CHEMBL4161877 IC50 = 457.0 nM 6.34