Assay Detail
Binding
CHEMBL4135538
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human full length HDAC1 expressed in baculovirus infected Sf9 insect cells using p53 (379 to 382 residues) derived RHKKAc as substrate preincubated for 5 to 10 mins followed by substrate addition measured after 2 hrs by fluorescence assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Synthesis and Pharmacological Evaluation of Selective Histone Deacetylase 6 Inhibitors in Melanoma Models.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 15 | 5.71 | 7.51 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL98 | VORINOSTAT | 4.0 | 1 | 7.51 |
| CHEMBL4166281 | — | — | 1 | 6.22 |
| CHEMBL4170768 | — | — | 1 | 6.14 |
| CHEMBL4162826 | — | — | 1 | 5.56 |
| CHEMBL4177129 | — | — | 1 | 5.54 |
| CHEMBL4166504 | — | — | 1 | 5.24 |
| CHEMBL4174193 | — | — | 1 | 5.21 |
| CHEMBL4167374 | — | — | 1 | 5.12 |
| CHEMBL4159465 | — | — | 1 | 4.84 |
| CHEMBL4162010 | — | — | 1 | - |
| CHEMBL4169931 | — | — | 1 | - |
| CHEMBL4165452 | — | — | 1 | - |
| CHEMBL4173367 | — | — | 1 | - |
| CHEMBL4168877 | — | — | 1 | - |
| CHEMBL4176731 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL98 | VORINOSTAT | IC50 | = | 31.0 | nM | 7.51 |
| CHEMBL4166281 | — | IC50 | = | 604.0 | nM | 6.22 |
| CHEMBL4170768 | — | IC50 | = | 721.0 | nM | 6.14 |
| CHEMBL4162826 | — | IC50 | = | 2740.0 | nM | 5.56 |
| CHEMBL4177129 | — | IC50 | = | 2913.0 | nM | 5.54 |
| CHEMBL4166504 | — | IC50 | = | 5740.0 | nM | 5.24 |
| CHEMBL4174193 | — | IC50 | = | 6130.0 | nM | 5.21 |
| CHEMBL4167374 | — | IC50 | = | 7540.0 | nM | 5.12 |
| CHEMBL4159465 | — | IC50 | = | 14400.0 | nM | 4.84 |
| CHEMBL4162010 | — | IC50 | > | 30000.0 | nM | - |
| CHEMBL4169931 | — | IC50 | - | — | - | |
| CHEMBL4165452 | — | IC50 | > | 30000.0 | nM | - |
| CHEMBL4173367 | — | IC50 | - | — | - | |
| CHEMBL4168877 | — | IC50 | - | — | - | |
| CHEMBL4176731 | — | IC50 | - | — | - |