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Assay Detail

CHEMBL4136597

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human His6-tagged USP28-5 (1 to 1077 residues) using Ub-Rh110 as substrate pretreated for 30 mins followed by substrate addition measured after 20 mins by fluorescence assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Ubiquitin-Specific Inhibitors for the Treatment of Cancers, Autoimmune, and Infectious Diseases.
Kargbo RB.
ACS Med Chem Lett (2017) 8 : 1211 -1212
PubMed 29259735 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 6.70 6.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4166283 1 6.70
CHEMBL4160957 1 6.70
CHEMBL4176483 1 6.70
CHEMBL4162425 1 6.70
CHEMBL4159041 1 6.70
CHEMBL4173102 1 -
CHEMBL4169696 1 -
CHEMBL4174196 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4166283 IC50 = 200.0 nM 6.70
CHEMBL4160957 IC50 = 200.0 nM 6.70
CHEMBL4176483 IC50 = 200.0 nM 6.70
CHEMBL4162425 IC50 = 200.0 nM 6.70
CHEMBL4159041 IC50 = 200.0 nM 6.70
CHEMBL4173102 IC50 < 200.0 nM -
CHEMBL4169696 IC50 < 200.0 nM -
CHEMBL4174196 IC50 < 200.0 nM -