Assay Detail
Binding
CHEMBL4136966
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at 6xHis tagged human RORgammat LBD (262 to 507 residues) expressed in Escherichia coli BL21 (DE3) assessed as biotinylated SRC1-2 peptide coactivator recruitment by AlphaScreen assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Intermediate |
Publication
Retinoic Acid Receptor-Related Orphan Receptor γt (RORγt) Agonists as Potential Small Molecule Therapeutics for Cancer Immunotherapy.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 4 | 7.70 | 7.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL169046 | — | — | 1 | 7.70 |
| CHEMBL422904 | 22(R)-HYDROXYCHOLESTEROL | — | 1 | 7.70 |
| CHEMBL1233249 | — | — | 1 | 7.70 |
| CHEMBL112570 | CHOLESTEROL | 2.0 | 1 | 7.70 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL169046 | — | EC50 | = | 20.0 | nM | 7.70 |
| CHEMBL422904 | 22(R)-HYDROXYCHOLESTEROL | EC50 | = | 20.0 | nM | 7.70 |
| CHEMBL1233249 | — | EC50 | = | 20.0 | nM | 7.70 |
| CHEMBL112570 | CHOLESTEROL | EC50 | = | 20.0 | nM | 7.70 |