Assay Detail
Binding
CHEMBL4141000
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human liver cathepsin D using Mca-Gly-Lys-Pro-Ile-Leu-PhePhe-Arg-Leu-Lys-(Dnp)-D-Arg-NH2 as substrate pretreated for 15 mins followed by substrate addition measured at 5 mins interval for 120 mins by fluorescence assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Grassystatins D-F, Potent Aspartic Protease Inhibitors from Marine Cyanobacteria as Potential Antimetastatic Agents Targeting Invasive Breast Cancer.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 9 | 7.17 | 9.46 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL296588 | PEPSTATIN | 2.0 | 1 | 9.46 |
| CHEMBL568553 | GRASSYSTATIN B | — | 1 | 8.14 |
| CHEMBL567893 | GRASSYSTATIN A | — | 1 | 7.58 |
| CHEMBL4167618 | — | — | 1 | 7.30 |
| CHEMBL4163078 | — | — | 1 | 7.30 |
| CHEMBL4159706 | — | — | 1 | 7.24 |
| CHEMBL4170992 | — | — | 1 | 6.05 |
| CHEMBL4174823 | — | — | 1 | 5.80 |
| CHEMBL4173516 | — | — | 1 | 5.70 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL296588 | PEPSTATIN | IC50 | = | 0.35 | nM | 9.46 |
| CHEMBL568553 | GRASSYSTATIN B | IC50 | = | 7.3 | nM | 8.14 |
| CHEMBL567893 | GRASSYSTATIN A | IC50 | = | 26.0 | nM | 7.58 |
| CHEMBL4167618 | — | IC50 | = | 50.0 | nM | 7.30 |
| CHEMBL4163078 | — | IC50 | = | 50.0 | nM | 7.30 |
| CHEMBL4159706 | — | IC50 | = | 57.0 | nM | 7.24 |
| CHEMBL4170992 | — | IC50 | = | 900.0 | nM | 6.05 |
| CHEMBL4174823 | — | IC50 | = | 1600.0 | nM | 5.80 |
| CHEMBL4173516 | — | IC50 | = | 2000.0 | nM | 5.70 |