Assay Detail
Binding
CHEMBL4141001
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human C-terminal His10-tagged cathepsin E (Gln18 to Pro396 residues) using Mca-Gly-Lys-Pro-Ile-Leu-PhePhe-Arg-Leu-Lys-(Dnp)-D-Arg-NH2 as substrate pretreated for 15 mins followed by substrate addition measured at 5 mins interval for 120 mins by fluorescence assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Grassystatins D-F, Potent Aspartic Protease Inhibitors from Marine Cyanobacteria as Potential Antimetastatic Agents Targeting Invasive Breast Cancer.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 9 | 8.46 | 9.52 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL296588 | PEPSTATIN | 2.0 | 1 | 9.52 |
| CHEMBL568553 | GRASSYSTATIN B | — | 1 | 9.40 |
| CHEMBL4163078 | — | — | 1 | 9.30 |
| CHEMBL567893 | GRASSYSTATIN A | — | 1 | 9.05 |
| CHEMBL4170992 | — | — | 1 | 8.30 |
| CHEMBL4167618 | — | — | 1 | 8.05 |
| CHEMBL4159706 | — | — | 1 | 7.64 |
| CHEMBL4173516 | — | — | 1 | 7.52 |
| CHEMBL4174823 | — | — | 1 | 7.37 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL296588 | PEPSTATIN | IC50 | = | 0.3 | nM | 9.52 |
| CHEMBL568553 | GRASSYSTATIN B | IC50 | = | 0.4 | nM | 9.40 |
| CHEMBL4163078 | — | IC50 | = | 0.5 | nM | 9.30 |
| CHEMBL567893 | GRASSYSTATIN A | IC50 | = | 0.9 | nM | 9.05 |
| CHEMBL4170992 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL4167618 | — | IC50 | = | 9.0 | nM | 8.05 |
| CHEMBL4159706 | — | IC50 | = | 23.0 | nM | 7.64 |
| CHEMBL4173516 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL4174823 | — | IC50 | = | 43.0 | nM | 7.37 |