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Assay Detail

CHEMBL4145136

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of protease L10F/V32I/M46I/I47V/Q58E/I84V mutant in HIV1 A17 infected in human MT4 cells assessed as reduction in virus-induced cytopathic effect after 5 days by MTT assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Human immunodeficiency virus 1
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Protease (CHEMBL2366517)
Type SINGLE PROTEIN
Organism Human immunodeficiency virus 1

Publication

Identification of Highly Potent Human Immunodeficiency Virus Type-1 Protease Inhibitors against Lopinavir and Darunavir Resistant Viruses from Allophenylnorstatine-Based Peptidomimetics with P2 Tetrahydrofuranylglycine.
Hidaka K, Kimura T, Sankaranarayanan R, Wang J, McDaniel KF, Kempf DJ, Kameoka M, Adachi M, Kuroki R, Nguyen JT, Hayashi Y, Kiso Y.
J Med Chem (2018) 61 : 5138 -5153
PubMed 29852069 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 11 6.59 7.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4173216 1 7.60
CHEMBL4164025 1 7.21
CHEMBL4160695 1 7.08
CHEMBL4163344 1 7.02
CHEMBL4168317 1 6.78
CHEMBL4160405 1 6.65
CHEMBL4165197 1 6.51
CHEMBL4159139 1 6.17
CHEMBL4160986 1 5.96
CHEMBL729 LOPINAVIR 4.0 1 5.94
CHEMBL300891 1 5.56

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4173216 EC50 = 25.0 nM 7.60
CHEMBL4164025 EC50 = 61.0 nM 7.21
CHEMBL4160695 EC50 = 84.0 nM 7.08
CHEMBL4163344 EC50 = 95.0 nM 7.02
CHEMBL4168317 EC50 = 165.0 nM 6.78
CHEMBL4160405 EC50 = 226.0 nM 6.65
CHEMBL4165197 EC50 = 309.0 nM 6.51
CHEMBL4159139 EC50 = 678.0 nM 6.17
CHEMBL4160986 EC50 = 1100.0 nM 5.96
CHEMBL729 LOPINAVIR EC50 = 1160.0 nM 5.94
CHEMBL300891 EC50 = 2730.0 nM 5.56