Assay Detail
Binding
CHEMBL4146154
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Mycobacterium tuberculosis H37Rv DHFR expressed in Escherichia coli assessed as reduction in consumption of NADPH using DHF as substrate by fluorescence method
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mycobacterium tuberculosis H37Rv |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Dihydrofolate reductase (CHEMBL6065) ↗| Type | SINGLE PROTEIN |
| Organism | Mycobacterium tuberculosis |
Publication
1,3,5-triazaspiro[5.5]undeca-2,4-dienes as selective Mycobacterium tuberculosis dihydrofolate reductase inhibitors with potent whole cell activity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 7.78 | 8.09 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL526013 | — | — | 1 | 8.09 |
| CHEMBL526243 | — | — | 1 | 8.00 |
| CHEMBL531405 | — | — | 1 | 7.26 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL526013 | — | IC50 | = | 8.2 | nM | 8.09 |
| CHEMBL526243 | — | IC50 | = | 10.1 | nM | 8.00 |
| CHEMBL531405 | — | IC50 | = | 54.5 | nM | 7.26 |