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Assay Detail

CHEMBL4146209

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant ALK L1196M mutant (unknown origin) using poly (Glu,Tyr) 4:1 as substrate incubated for 60 mins by ELISA
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

ALK tyrosine kinase receptor (CHEMBL4247)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 2,4-diarylaminopyrimidines bearing a resorcinol motif as novel ALK inhibitors to overcome the G1202R resistant mutation.
Geng K, Xia Z, Ji Y, Zhang RR, Sun D, Ai J, Song Z, Geng M, Zhang A.
Eur J Med Chem (2018) 144 : 386 -397
PubMed 29288940 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 7.63 8.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4168868 1 8.46
CHEMBL4172048 1 8.30
CHEMBL4177038 1 7.85
CHEMBL4174031 1 7.09
CHEMBL601719 CRIZOTINIB 4.0 1 6.45

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4168868 IC50 = 3.5 nM 8.46
CHEMBL4172048 IC50 = 5.0 nM 8.30
CHEMBL4177038 IC50 = 14.0 nM 7.85
CHEMBL4174031 IC50 = 81.0 nM 7.09
CHEMBL601719 CRIZOTINIB IC50 = 355.0 nM 6.45