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Assay Detail

CHEMBL4146975

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]D-Trp6-LHRH from human GnRH receptor expressed in CHO-K1 cells after 1 hr by microbeta scintillation counting method
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Gonadotropin-releasing hormone receptor (CHEMBL1855)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological evaluation of 3-(2-aminoethyl) uracil derivatives as gonadotropin-releasing hormone (GnRH) receptor antagonists.
Kim SM, Lee M, Lee SY, Lee SM, Kim EJ, Kim JS, Ann J, Lee J, Lee J.
Eur J Med Chem (2018) 145 : 413 -424
PubMed 29335207 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 8.29 9.54

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4159155 1 9.54
CHEMBL4169891 1 9.48
CHEMBL4165983 1 9.42
CHEMBL4173065 1 9.34
CHEMBL4159247 1 9.24
CHEMBL179691 1 9.23
CHEMBL4170478 1 9.13
CHEMBL4167067 1 8.92
CHEMBL4163619 1 8.64
CHEMBL4173914 1 8.27
CHEMBL4160683 1 8.21
CHEMBL4169027 1 8.12
CHEMBL4171288 1 8.05
CHEMBL4175801 1 7.95
CHEMBL4164508 1 7.80
CHEMBL4166009 1 7.76
CHEMBL4161096 1 7.72
CHEMBL4162543 1 7.69
CHEMBL4172391 1 7.34
CHEMBL4164417 1 7.30
CHEMBL4167945 1 7.21
CHEMBL4170863 1 7.16
CHEMBL4174298 1 7.13
CHEMBL4160259 1 -
CHEMBL4175063 1 -
CHEMBL4172747 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4159155 IC50 = 0.29 nM 9.54
CHEMBL4169891 IC50 = 0.33 nM 9.48
CHEMBL4165983 IC50 = 0.38 nM 9.42
CHEMBL4173065 IC50 = 0.46 nM 9.34
CHEMBL4159247 IC50 = 0.58 nM 9.24
CHEMBL179691 IC50 = 0.59 nM 9.23
CHEMBL4170478 IC50 = 0.74 nM 9.13
CHEMBL4167067 IC50 = 1.21 nM 8.92
CHEMBL4163619 IC50 = 2.3 nM 8.64
CHEMBL4173914 IC50 = 5.4 nM 8.27
CHEMBL4160683 IC50 = 6.2 nM 8.21
CHEMBL4169027 IC50 = 7.5 nM 8.12
CHEMBL4171288 IC50 = 9.0 nM 8.05
CHEMBL4175801 IC50 = 11.3 nM 7.95
CHEMBL4164508 IC50 = 16.0 nM 7.80
CHEMBL4166009 IC50 = 17.2 nM 7.76
CHEMBL4161096 IC50 = 19.1 nM 7.72
CHEMBL4162543 IC50 = 20.4 nM 7.69
CHEMBL4172391 IC50 = 46.0 nM 7.34
CHEMBL4164417 IC50 = 49.5 nM 7.30
CHEMBL4167945 IC50 = 62.3 nM 7.21
CHEMBL4170863 IC50 = 69.1 nM 7.16
CHEMBL4174298 IC50 = 73.3 nM 7.13
CHEMBL4160259 IC50 > 1000.0 nM -
CHEMBL4175063 IC50 > 100.0 nM -
CHEMBL4172747 IC50 > 1000.0 nM -