Assay Detail
Binding
CHEMBL4147367
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of bacterial C-terminal His6-tagged ErmC' expressed in Escherichia coli BL21(DE3) assessed as decrease of protein binding to [gamma-33P]ATP-labeled 32-nt oligonucleotide after 30 mins by filter binding assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Bacteria |
| Confidence | 8 — Homologous single protein target |
| Curated By | Intermediate |
Target
rRNA adenine N-6-methyltransferase (CHEMBL4295568) ↗| Type | SINGLE PROTEIN |
| Organism | Staphylococcus aureus (strain N315) |
Publication
Novel inhibitors of the rRNA ErmC' methyltransferase to block resistance to macrolides, lincosamides, streptogramine B antibiotics.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 2 | 6.69 | 7.40 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4177311 | — | — | 1 | 7.40 |
| CHEMBL4166678 | — | — | 1 | 5.98 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4177311 | — | Kd | = | 40.0 | nM | 7.40 |
| CHEMBL4166678 | — | Kd | = | 1040.0 | nM | 5.98 |