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Assay Detail

CHEMBL4149306

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of TNFalpha stimulated NF-kappaB (unknown origin) expressed in HEK293 cells after 5 hrs by luciferase reporter gene based luminescence assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Carbazole-, Aspidofractinine-, and Aspidocarpamine-Type Alkaloids from Pleiocarpa pycnantha.
Ndongo JT, Mbing JN, Monteillier A, Tala MF, Rütten M, Mombers D, Cuendet M, Pegnyemb DE, Dittrich B, Laatsch H.
J Nat Prod (2018) 81 : 1193 -1202
PubMed 29664292 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 5.29 6.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4298697 1 6.40
CHEMBL4176582 1 5.08
CHEMBL4159999 1 5.06
CHEMBL4159200 1 5.03
CHEMBL4168718 1 4.88
CHEMBL4167918 1 -
CHEMBL2229719 1 -
CHEMBL4173934 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4298697 IC50 = 400.0 nM 6.40
CHEMBL4176582 IC50 = 8400.0 nM 5.08
CHEMBL4159999 IC50 = 8800.0 nM 5.06
CHEMBL4159200 IC50 = 9400.0 nM 5.03
CHEMBL4168718 IC50 = 13100.0 nM 4.88
CHEMBL4167918 IC50 > 50000.0 nM -
CHEMBL2229719 IC50 > 50000.0 nM -
CHEMBL4173934 IC50 > 50000.0 nM -