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Assay Detail

CHEMBL4150585

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length human recombinant HDAC1 expressed in baculovirus expression system using (Ac-Leu-Gly-Lys(Ac)-AMC as substrate preincubated for 5 mins followed by substrate addition and measured after 30 mins by fluorescence assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 1 (CHEMBL325)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Janus Kinase 2 (JAK2) and Histone Deacetylase (HDAC) Dual Inhibitors as a Novel Strategy for the Combinational Treatment of Leukemia and Invasive Fungal Infections.
Huang Y, Dong G, Li H, Liu N, Zhang W, Sheng C.
J Med Chem (2018) 61 : 6056 -6074
PubMed 29940115 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 6.07 7.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4159366 1 7.75
CHEMBL98 VORINOSTAT 4.0 1 7.40
CHEMBL4160840 1 6.80
CHEMBL4167286 1 6.60
CHEMBL4175555 1 6.60
CHEMBL4160833 1 6.46
CHEMBL4167685 1 6.42
CHEMBL4165344 1 5.96
CHEMBL4170168 1 5.77
CHEMBL4164238 1 5.68
CHEMBL4168763 1 5.66
CHEMBL4162254 1 5.57
CHEMBL4172110 1 5.54
CHEMBL4169802 1 5.29
CHEMBL4175964 1 5.04
CHEMBL4168707 1 4.62
CHEMBL4168767 1 -
CHEMBL1078178 MOMELOTINIB 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4159366 IC50 = 18.0 nM 7.75
CHEMBL98 VORINOSTAT IC50 = 40.0 nM 7.40
CHEMBL4160840 IC50 = 160.0 nM 6.80
CHEMBL4167286 IC50 = 250.0 nM 6.60
CHEMBL4175555 IC50 = 250.0 nM 6.60
CHEMBL4160833 IC50 = 350.0 nM 6.46
CHEMBL4167685 IC50 = 380.0 nM 6.42
CHEMBL4165344 IC50 = 1100.0 nM 5.96
CHEMBL4170168 IC50 = 1700.0 nM 5.77
CHEMBL4164238 IC50 = 2100.0 nM 5.68
CHEMBL4168763 IC50 = 2200.0 nM 5.66
CHEMBL4162254 IC50 = 2700.0 nM 5.57
CHEMBL4172110 IC50 = 2900.0 nM 5.54
CHEMBL4169802 IC50 = 5100.0 nM 5.29
CHEMBL4175964 IC50 = 9200.0 nM 5.04
CHEMBL4168707 IC50 = 24000.0 nM 4.62
CHEMBL4168767 IC50 > 50000.0 nM -
CHEMBL1078178 MOMELOTINIB IC50 - -