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Assay Detail

CHEMBL4150869

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release preincubated for 5 mins followed by fMLP/CB stimulation and measured for 10 mins using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type Neutrophil
Confidence 1 — Organism
Curated By Intermediate

Target

Neutrophil (CHEMBL5314326)
Type CELL-LINE
Organism Homo sapiens

Publication

Bioactive Phenolic Components from the Twigs of Atalantia buxifolia.
Chang FR, Li PS, Huang Liu R, Hu HC, Hwang TL, Lee JC, Chen SL, Wu YC, Cheng YB.
J Nat Prod (2018) 81 : 1534 -1539
PubMed 29975532 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 5.36 5.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL452222 1 5.43
CHEMBL98350 LY-294002 -1.0 1 5.29
CHEMBL71358 SESALIN 1 -
CHEMBL448060 ISOIMPERATORIN 1 -
CHEMBL453805 PENTOSALEN -1.0 1 -
CHEMBL4159088 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL452222 IC50 = 3700.0 nM 5.43
CHEMBL98350 LY-294002 IC50 = 5100.0 nM 5.29
CHEMBL71358 SESALIN IC50 > 10000.0 nM -
CHEMBL448060 ISOIMPERATORIN IC50 > 10000.0 nM -
CHEMBL453805 PENTOSALEN IC50 > 10000.0 nM -
CHEMBL4159088 IC50 > 10000.0 nM -