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Assay Detail

CHEMBL4152128

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Keap1 Kelch domain (unknown origin) /Nrf2 (unknown origin) interaction using fluorescein-labeled 9-mer peptide containing NRF2 Neh2 domain ETGE motif by fluorescence anisotropy assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Intermediate

Target

Keap1/Nrf2 (CHEMBL3038498)
Type PROTEIN-PROTEIN INTERACTION
Organism Homo sapiens

Publication

Replacement of a Naphthalene Scaffold in Kelch-like ECH-Associated Protein 1 (KEAP1)/Nuclear Factor (Erythroid-derived 2)-like 2 (NRF2) Inhibitors.
Richardson BG, Jain AD, Potteti HR, Lazzara PR, David BP, Tamatam CR, Choma E, Skowron K, Dye K, Siddiqui Z, Wang YT, Krunic A, Reddy SP, Moore TW.
J Med Chem (2018) 61 : 8029 -8047
PubMed 30122040 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 6.61 7.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3237245 1 7.60
CHEMBL4174651 1 7.22
CHEMBL3632711 1 7.20
CHEMBL4166445 1 7.00
CHEMBL2402207 1 6.03
CHEMBL3600957 1 6.01
CHEMBL4173296 1 5.96
CHEMBL4169869 1 5.89
CHEMBL4160703 1 -
CHEMBL4176664 1 -
CHEMBL4166048 1 -
CHEMBL4162602 1 -
CHEMBL4159226 1 -
CHEMBL4171299 1 -
CHEMBL4166752 1 -
CHEMBL4176126 1 -
CHEMBL3632890 1 -
CHEMBL4174360 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3237245 IC50 = 25.0 nM 7.60
CHEMBL4174651 IC50 = 60.0 nM 7.22
CHEMBL3632711 IC50 = 63.0 nM 7.20
CHEMBL4166445 IC50 = 101.0 nM 7.00
CHEMBL2402207 IC50 = 940.0 nM 6.03
CHEMBL3600957 IC50 = 980.0 nM 6.01
CHEMBL4173296 IC50 = 1100.0 nM 5.96
CHEMBL4169869 IC50 = 1300.0 nM 5.89
CHEMBL4160703 IC50 > 25000.0 nM -
CHEMBL4176664 IC50 > 25000.0 nM -
CHEMBL4166048 IC50 > 25000.0 nM -
CHEMBL4162602 IC50 > 25000.0 nM -
CHEMBL4159226 IC50 > 25000.0 nM -
CHEMBL4171299 IC50 > 25000.0 nM -
CHEMBL4166752 IC50 - -
CHEMBL4176126 IC50 > 25000.0 nM -
CHEMBL3632890 IC50 > 25000.0 nM -
CHEMBL4174360 IC50 > 25000.0 nM -