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Assay Detail

CHEMBL4153210

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at P2X7 in LPS/IFN gamma differentiated human THP-1 cells assessed as inhibition of BzATP-induced IL-1beta release preincubated for 30 mins followed by BzATP stimulation for 30 mins by ELISA
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type THP-1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2X purinoceptor 7 (CHEMBL4805)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and structure-activity relationships of quinolinone and quinoline-based P2X7 receptor antagonists and their anti-sphere formation activities in glioblastoma cells.
Kwak SH, Shin S, Lee JH, Shim JK, Kim M, Lee SD, Lee A, Bae J, Park JH, Abdelrahman A, Müller CE, Cho SK, Kang SG, Bae MA, Yang JY, Ko H, Goddard WA, Kim YC.
Eur J Med Chem (2018) 151 : 462 -481
PubMed 29649742 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.43 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4171354 1 8.52
CHEMBL4175330 1 8.22
CHEMBL4175875 1 8.15
CHEMBL4159914 1 7.92
CHEMBL4163225 1 7.72
CHEMBL4169592 1 7.60
CHEMBL4159276 1 7.54
CHEMBL4159289 1 7.48
CHEMBL4176511 1 6.95
CHEMBL28324 1 6.89
CHEMBL4165201 1 6.75
CHEMBL4160756 1 6.47
CHEMBL4167190 1 6.36
CHEMBL4161406 1 -
CHEMBL4167598 1 -
CHEMBL4172722 1 -
CHEMBL4168674 1 -
CHEMBL4165253 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4171354 IC50 = 3.0 nM 8.52
CHEMBL4175330 IC50 = 6.0 nM 8.22
CHEMBL4175875 IC50 = 7.0 nM 8.15
CHEMBL4159914 IC50 = 12.0 nM 7.92
CHEMBL4163225 IC50 = 19.0 nM 7.72
CHEMBL4169592 IC50 = 25.0 nM 7.60
CHEMBL4159276 IC50 = 29.0 nM 7.54
CHEMBL4159289 IC50 = 33.0 nM 7.48
CHEMBL4176511 IC50 = 113.0 nM 6.95
CHEMBL28324 IC50 = 130.0 nM 6.89
CHEMBL4165201 IC50 = 178.0 nM 6.75
CHEMBL4160756 IC50 = 335.0 nM 6.47
CHEMBL4167190 IC50 = 434.0 nM 6.36
CHEMBL4161406 IC50 - -
CHEMBL4167598 IC50 - -
CHEMBL4172722 IC50 - -
CHEMBL4168674 IC50 - -
CHEMBL4165253 IC50 - -