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Assay Detail

CHEMBL4153246

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human P2X7 receptor in expressed in HEK293 cells assessed as inhibition of BzATP-induced EtBr uptake
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2X purinoceptor 7 (CHEMBL4805)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and structure-activity relationships of quinolinone and quinoline-based P2X7 receptor antagonists and their anti-sphere formation activities in glioblastoma cells.
Kwak SH, Shin S, Lee JH, Shim JK, Kim M, Lee SD, Lee A, Bae J, Park JH, Abdelrahman A, Müller CE, Cho SK, Kang SG, Bae MA, Yang JY, Ko H, Goddard WA, Kim YC.
Eur J Med Chem (2018) 151 : 462 -481
PubMed 29649742 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 7.84 9.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4165149 1 9.96
CHEMBL4171354 1 8.82
CHEMBL1823817 CE-224535 2.0 1 8.40
CHEMBL2048438 1 7.89
CHEMBL4173050 1 7.27
CHEMBL4169645 1 7.07
CHEMBL28324 1 6.80
CHEMBL377219 1 6.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4165149 IC50 = 0.11 nM 9.96
CHEMBL4171354 IC50 = 1.5 nM 8.82
CHEMBL1823817 CE-224535 IC50 = 4.0 nM 8.40
CHEMBL2048438 IC50 = 13.0 nM 7.89
CHEMBL4173050 IC50 = 54.0 nM 7.27
CHEMBL4169645 IC50 = 85.0 nM 7.07
CHEMBL28324 IC50 = 158.0 nM 6.80
CHEMBL377219 IC50 = 300.0 nM 6.52