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Assay Detail

CHEMBL4153627

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Binding
Inhibition of yeast GCN5 (99 to 262 residues) expressed in Escherichia coli BL21 (DE3) preincubated for 15 mins followed by biotin tagged H3 peptide ( 1 to 23 residues) and [3H]-Ac-CoA addition and measured after 60 mins by radioactive acetylation assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Saccharomyces cerevisiae
Confidence 8 — Homologous single protein target
Curated By Intermediate

Target

Histone acetyltransferase GCN5 (CHEMBL4669)
Type SINGLE PROTEIN
Organism Saccharomyces cerevisiae S288c

Publication

Discovery of 1,8-acridinedione derivatives as novel GCN5 inhibitors via high throughput screening.
Xiong H, Han J, Wang J, Lu W, Wang C, Chen Y, Fulin Lian, Zhang N, Liu YC, Zhang C, Ding H, Jiang H, Lu W, Luo C, Zhou B.
Eur J Med Chem (2018) 151 : 740 -751
PubMed 29665527 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 4.75 5.54

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1365300 1 5.54
CHEMBL4168633 1 5.17
CHEMBL4172975 1 5.07
CHEMBL4171987 1 4.80
CHEMBL4160712 1 4.72
CHEMBL4159754 1 4.70
CHEMBL4175431 1 4.51
CHEMBL4162185 1 4.47
CHEMBL4165080 1 4.46
CHEMBL4170095 1 4.09
CHEMBL4167662 1 -
CHEMBL4175827 1 -
CHEMBL4165213 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1365300 IC50 = 2900.0 nM 5.54
CHEMBL4168633 IC50 = 6800.0 nM 5.17
CHEMBL4172975 IC50 = 8600.0 nM 5.07
CHEMBL4171987 IC50 = 16000.0 nM 4.80
CHEMBL4160712 IC50 = 19000.0 nM 4.72
CHEMBL4159754 IC50 = 20000.0 nM 4.70
CHEMBL4175431 IC50 = 31000.0 nM 4.51
CHEMBL4162185 IC50 = 34000.0 nM 4.47
CHEMBL4165080 IC50 = 34700.0 nM 4.46
CHEMBL4170095 IC50 = 81000.0 nM 4.09
CHEMBL4167662 IC50 - -
CHEMBL4175827 IC50 - -
CHEMBL4165213 IC50 - -