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Assay Detail

CHEMBL4153771

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ATR in human HT29 cells assessed as decrease in Chk1 phosphorylation at Ser 345 preincubated for 1 hr followed by 4NQO addition and measured after 1 hr by Hoechst 33342 dye-based immunofluorescence assay
40
Total Activities
40
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase ATR (CHEMBL5024)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The Identification of Potent, Selective, and Orally Available Inhibitors of Ataxia Telangiectasia Mutated (ATM) Kinase: The Discovery of AZD0156 (8-{6-[3-(Dimethylamino)propoxy]pyridin-3-yl}-3-methyl-1-(tetrahydro-2 H-pyran-4-yl)-1,3-dihydro-2 H-imidazo[4,5- c]quinolin-2-one).
Pike KG, Barlaam B, Cadogan E, Campbell A, Chen Y, Colclough N, Davies NL, de-Almeida C, Degorce SL, Didelot M, Dishington A, Ducray R, Durant ST, Hassall LA, Holmes J, Hughes GD, MacFaul PA, Mulholland KR, McGuire TM, Ouvry G, Pass M, Robb G, Stratton N, Wang Z, Wilson J, Zhai B, Zhao K, Al-Huniti N.
J Med Chem (2018) 61 : 3823 -3841
PubMed 29683659 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 40 5.74 7.29

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4159181 1 7.29
CHEMBL4169828 1 7.06
CHEMBL4163629 1 5.70
CHEMBL4174323 1 5.51
CHEMBL3954517 1 5.36
CHEMBL3960662 1 5.21
CHEMBL4162142 1 5.00
CHEMBL4164853 1 4.82
CHEMBL4167508 1 -
CHEMBL4164430 1 -
CHEMBL3946515 1 -
CHEMBL4172819 1 -
CHEMBL4170883 1 -
CHEMBL3898577 1 -
CHEMBL3950905 1 -
CHEMBL4173181 1 -
CHEMBL4172761 1 -
CHEMBL4161022 1 -
CHEMBL4171626 1 -
CHEMBL4159593 1 -
CHEMBL4111966 1 -
CHEMBL3890490 1 -
CHEMBL3972724 1 -
CHEMBL4176012 1 -
CHEMBL4173494 1 -
CHEMBL3960323 1 -
CHEMBL3936943 1 -
CHEMBL3979811 1 -
CHEMBL3958682 1 -
CHEMBL3889770 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4159181 IC50 = 51.0 nM 7.29
CHEMBL4169828 IC50 = 87.0 nM 7.06
CHEMBL4163629 IC50 = 2000.0 nM 5.70
CHEMBL4174323 IC50 = 3100.0 nM 5.51
CHEMBL3954517 IC50 = 4400.0 nM 5.36
CHEMBL3960662 IC50 = 6200.0 nM 5.21
CHEMBL4162142 IC50 = 10000.0 nM 5.00
CHEMBL4164853 IC50 = 15000.0 nM 4.82
CHEMBL3950905 IC50 > 30000.0 nM -
CHEMBL3924247 IC50 > 30000.0 nM -
CHEMBL4173181 IC50 > 30000.0 nM -
CHEMBL4172761 IC50 > 16000.0 nM -
CHEMBL4161022 IC50 > 30000.0 nM -
CHEMBL4171626 IC50 > 30000.0 nM -
CHEMBL4164430 IC50 > 30000.0 nM -
CHEMBL4175071 IC50 > 30000.0 nM -
CHEMBL3936734 IC50 > 30000.0 nM -
CHEMBL3986918 IC50 > 30000.0 nM -
CHEMBL4162952 IC50 > 20000.0 nM -
CHEMBL4167277 IC50 > 30000.0 nM -
CHEMBL4176135 IC50 > 30000.0 nM -
CHEMBL4168290 IC50 > 28000.0 nM -
CHEMBL4160664 IC50 > 30000.0 nM -
CHEMBL4171264 IC50 > 26000.0 nM -
CHEMBL3898577 IC50 > 30000.0 nM -
CHEMBL4170883 IC50 > 30000.0 nM -
CHEMBL4172819 IC50 > 30000.0 nM -
CHEMBL3946515 IC50 > 29000.0 nM -
CHEMBL4167508 IC50 > 13000.0 nM -
CHEMBL4159593 IC50 > 27600.0 nM -
CHEMBL4111966 IC50 > 23000.0 nM -
CHEMBL3890490 IC50 > 30000.0 nM -
CHEMBL3972724 IC50 > 30000.0 nM -
CHEMBL4176012 IC50 > 30000.0 nM -
CHEMBL4173494 IC50 > 30000.0 nM -
CHEMBL3960323 IC50 > 2600.0 nM -
CHEMBL3936943 IC50 > 24000.0 nM -
CHEMBL3979811 IC50 > 24000.0 nM -
CHEMBL3958682 IC50 > 30000.0 nM -
CHEMBL3889770 IC50 > 30000.0 nM -