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Assay Detail

CHEMBL4154039

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to N-terminal His6-tagged p53 DNA binding domain Y220C mutant (94 to 312 residues) (unknown origin) expressed in Escherichia coli C41 by ITC
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Cellular tumor antigen p53 (CHEMBL4096)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Aminobenzothiazole derivatives stabilize the thermolabile p53 cancer mutant Y220C and show anticancer activity in p53-Y220C cell lines.
Baud MGJ, Bauer MR, Verduci L, Dingler FA, Patel KJ, Horil Roy D, Joerger AC, Fersht AR.
Eur J Med Chem (2018) 152 : 101 -114
PubMed 29702446 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 20 4.54 5.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4162051 1 5.40
CHEMBL4168439 1 4.85
CHEMBL4165245 1 4.68
CHEMBL4168045 1 4.66
CHEMBL4176302 1 4.52
CHEMBL4164612 1 4.52
CHEMBL4168664 1 4.52
CHEMBL4160123 1 4.48
CHEMBL4164831 1 4.30
CHEMBL4175464 1 4.22
CHEMBL4163490 1 4.20
CHEMBL4176523 1 4.16
CHEMBL4171387 1 -
CHEMBL4168268 1 -
CHEMBL4160358 1 -
CHEMBL4161418 1 -
CHEMBL4171145 1 -
CHEMBL4175243 1 -
CHEMBL4161192 1 -
CHEMBL4171798 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4162051 Kd = 4000.0 nM 5.40
CHEMBL4168439 Kd = 14000.0 nM 4.85
CHEMBL4165245 Kd = 21000.0 nM 4.68
CHEMBL4168045 Kd = 22000.0 nM 4.66
CHEMBL4176302 Kd = 30000.0 nM 4.52
CHEMBL4164612 Kd = 30000.0 nM 4.52
CHEMBL4168664 Kd = 30000.0 nM 4.52
CHEMBL4160123 Kd = 33000.0 nM 4.48
CHEMBL4164831 Kd = 50000.0 nM 4.30
CHEMBL4175464 Kd = 60000.0 nM 4.22
CHEMBL4163490 Kd = 63000.0 nM 4.20
CHEMBL4176523 Kd = 70000.0 nM 4.16
CHEMBL4171387 Kd = 122000.0 nM -
CHEMBL4168268 Kd = 113000.0 nM -
CHEMBL4160358 Kd = 306000.0 nM -
CHEMBL4161418 Kd = 139000.0 nM -
CHEMBL4171145 Kd - -
CHEMBL4175243 Kd = 108000.0 nM -
CHEMBL4161192 Kd = 726000.0 nM -
CHEMBL4171798 Kd = 116000.0 nM -