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Assay Detail

CHEMBL4154378

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at human GPR109a expressed in HTLA cells assessed as increase in beta-arrestin2 recruitment after overnight incubation by Tango assay
17
Total Activities
6
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Hydroxycarboxylic acid receptor 2 (CHEMBL3785)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of coumarin-dihydroquinazolinone analogs as niacin receptor 1 agonist with in-vivo anti-obesity efficacy.
Singh LR, Kumar A, Upadhyay A, Gupta S, Palanati GR, Sikka K, Siddiqi MI, Yadav PN, Sashidhara KV.
Eur J Med Chem (2018) 152 : 208 -222
PubMed 29709786 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 12 6.14 6.63
Activity 5 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4174798 3 6.63
CHEMBL4163051 3 6.56
CHEMBL4159670 3 6.34
CHEMBL4167588 3 6.00
CHEMBL4164140 3 5.77
CHEMBL573 NIACIN 4.0 2 5.56

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4174798 EC50 = 232.0 nM 6.63
CHEMBL4174798 EC50 = 234.42 nM 6.63
CHEMBL4163051 EC50 = 275.42 nM 6.56
CHEMBL4163051 EC50 = 276.0 nM 6.56
CHEMBL4159670 EC50 = 457.09 nM 6.34
CHEMBL4159670 EC50 = 454.0 nM 6.34
CHEMBL4167588 EC50 = 1005.0 nM 6.00
CHEMBL4167588 EC50 = 1023.29 nM 5.99
CHEMBL4164140 EC50 = 1698.24 nM 5.77
CHEMBL4164140 EC50 = 1686.0 nM 5.77
CHEMBL573 NIACIN EC50 = 2727.0 nM 5.56
CHEMBL573 NIACIN EC50 = 2754.23 nM 5.56
CHEMBL4174798 Activity - -
CHEMBL4164140 Activity - -
CHEMBL4159670 Activity - -
CHEMBL4167588 Activity - -
CHEMBL4163051 Activity - -