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Assay Detail

CHEMBL4156074

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inverse agonist activity at human adenosine A2B receptor expressed in CHO cells assessed as inhibition of cAMP accumulation measured after 150 mins in presence of [3H]cAMP by scintillation counting method
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A2b (CHEMBL255)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-activity relationship studies and pharmacological characterization of N5-heteroarylalkyl-substituted-2-(2-furanyl)thiazolo[5,4-d]pyrimidine-5,7-diamine-based derivatives as inverse agonists at human A2A adenosine receptor.
Varano F, Catarzi D, Vincenzi F, Falsini M, Pasquini S, Borea PA, Colotta V, Varani K.
Eur J Med Chem (2018) 155 : 552 -561
PubMed 29909340 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 7.04 8.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4162638 1 8.38
CHEMBL4159215 1 8.05
CHEMBL4172818 1 7.88
CHEMBL4167557 1 7.85
CHEMBL4175676 1 7.48
CHEMBL113142 ZM-241385 1 7.29
CHEMBL4161758 1 7.12
CHEMBL4172399 1 7.09
CHEMBL4172715 1 7.04
CHEMBL4167813 1 7.02
CHEMBL4167125 1 6.96
CHEMBL4172264 1 6.95
CHEMBL4165484 1 6.92
CHEMBL4168980 1 6.41
CHEMBL4168016 1 6.26
CHEMBL4175878 1 5.56
CHEMBL4162037 1 5.34

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4162638 IC50 = 4.21 nM 8.38
CHEMBL4159215 IC50 = 8.96 nM 8.05
CHEMBL4172818 IC50 = 13.2 nM 7.88
CHEMBL4167557 IC50 = 14.0 nM 7.85
CHEMBL4175676 IC50 = 33.0 nM 7.48
CHEMBL113142 ZM-241385 IC50 = 51.0 nM 7.29
CHEMBL4161758 IC50 = 75.0 nM 7.12
CHEMBL4172399 IC50 = 82.0 nM 7.09
CHEMBL4172715 IC50 = 91.0 nM 7.04
CHEMBL4167813 IC50 = 95.0 nM 7.02
CHEMBL4167125 IC50 = 109.0 nM 6.96
CHEMBL4172264 IC50 = 112.0 nM 6.95
CHEMBL4165484 IC50 = 120.0 nM 6.92
CHEMBL4168980 IC50 = 389.0 nM 6.41
CHEMBL4168016 IC50 = 547.0 nM 6.26
CHEMBL4175878 IC50 = 2773.0 nM 5.56
CHEMBL4162037 IC50 = 4571.0 nM 5.34