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Assay Detail

CHEMBL4156811

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at CXCR4 in human CCRF-CEM cells assessed as inhibition of SDF-1alpha-induced calcium release preincubated for 25 mins followed by SDF-1alpha stimulation and measured for 90 secs by calcium flux assay
34
Total Activities
34
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CCRF-CEM
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

C-X-C chemokine receptor type 4 (CHEMBL2107)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, Synthesis, and Pharmacological Evaluation of Second-Generation Tetrahydroisoquinoline-Based CXCR4 Antagonists with Favorable ADME Properties.
Nguyen HH, Kim MB, Wilson RJ, Butch CJ, Kuo KM, Miller EJ, Tahirovic YA, Jecs E, Truax VM, Wang T, Sum CS, Cvijic ME, Schroeder GM, Wilson LJ, Liotta DC.
J Med Chem (2018) 61 : 7168 -7188
PubMed 30052039 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 34 7.39 8.54

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4173977 1 8.54
CHEMBL4162609 1 8.45
CHEMBL4159234 1 8.35
CHEMBL4172669 1 8.22
CHEMBL3091687 1 8.20
CHEMBL4163874 1 7.86
CHEMBL4170542 1 7.85
CHEMBL4169280 1 7.83
CHEMBL4176701 1 7.79
CHEMBL4171755 1 7.77
CHEMBL4169875 1 7.75
CHEMBL4163467 1 7.68
CHEMBL4161589 1 7.67
CHEMBL4167324 1 7.63
CHEMBL4171365 1 7.60
CHEMBL4165854 1 7.57
CHEMBL4168391 1 7.53
CHEMBL4167145 1 7.51
CHEMBL4166059 1 7.50
CHEMBL4177130 1 7.49
CHEMBL4164970 1 7.47
CHEMBL4160496 1 7.21
CHEMBL4169504 1 7.04
CHEMBL4175596 1 6.84
CHEMBL4176403 1 6.81
CHEMBL4177338 1 6.77
CHEMBL4172898 1 6.71
CHEMBL4162011 1 6.50
CHEMBL4173777 1 6.44
CHEMBL4175201 1 6.42

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4173977 IC50 = 2.92 nM 8.54
CHEMBL4162609 IC50 = 3.59 nM 8.45
CHEMBL4159234 IC50 = 4.47 nM 8.35
CHEMBL4172669 IC50 = 6.08 nM 8.22
CHEMBL3091687 IC50 = 6.25 nM 8.20
CHEMBL4163874 IC50 = 13.8 nM 7.86
CHEMBL4170542 IC50 = 14.1 nM 7.85
CHEMBL4169280 IC50 = 14.8 nM 7.83
CHEMBL4176701 IC50 = 16.1 nM 7.79
CHEMBL4171755 IC50 = 16.9 nM 7.77
CHEMBL4169875 IC50 = 17.9 nM 7.75
CHEMBL4163467 IC50 = 20.7 nM 7.68
CHEMBL4161589 IC50 = 21.4 nM 7.67
CHEMBL4167324 IC50 = 23.5 nM 7.63
CHEMBL4171365 IC50 = 25.2 nM 7.60
CHEMBL4165854 IC50 = 26.7 nM 7.57
CHEMBL4168391 IC50 = 29.6 nM 7.53
CHEMBL4167145 IC50 = 30.7 nM 7.51
CHEMBL4166059 IC50 = 32.0 nM 7.50
CHEMBL4177130 IC50 = 32.2 nM 7.49
CHEMBL4164970 IC50 = 33.9 nM 7.47
CHEMBL4160496 IC50 = 61.0 nM 7.21
CHEMBL4169504 IC50 = 91.2 nM 7.04
CHEMBL4175596 IC50 = 146.0 nM 6.84
CHEMBL4176403 IC50 = 154.0 nM 6.81
CHEMBL4177338 IC50 = 171.0 nM 6.77
CHEMBL4172898 IC50 = 195.0 nM 6.71
CHEMBL4162011 IC50 = 313.0 nM 6.50
CHEMBL4173777 IC50 = 364.0 nM 6.44
CHEMBL4175201 IC50 = 381.0 nM 6.42
CHEMBL4168418 IC50 = 498.0 nM 6.30
CHEMBL4166081 IC50 = 510.0 nM 6.29
CHEMBL4176285 IC50 = 715.0 nM 6.15
CHEMBL4161949 IC50 > 16700.0 nM -