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Assay Detail

CHEMBL4157891

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antioxidant activity assessed as protection against ferrous ions and dioxygen-induced free radical-mediated DNA strand break in pEMBL8 supercoiled DNA preincubated with DNA followed by (NH4)2Fe(SO4)2.6H2O addition measured after 6 mins by ethidium bromide staining based agarose gel electrophoresis method
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Confidence 3 — Cell-line / Tissue
Curated By Autocuration

Target

DNA (CHEMBL2364041)
Type NUCLEIC-ACID
Organism Bacteria

Publication

Synthesis and biological evaluation of novel heteroring-annulated pyrrolino-tetrahydroberberine analogues as antioxidant agents.
Mari G, Catalani S, Antonini E, De Crescentini L, Mantellini F, Santeusanio S, Lombardi P, Amicucci A, Battistelli S, Benedetti S, Palma F.
Bioorg Med Chem (2018) 26 : 5037 -5044
PubMed 30196978 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 8 4.12 4.20

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4175741 1 4.20
CHEMBL4172332 1 4.13
CHEMBL275097 TETRAHYDROBERBERINE 1 4.03
CHEMBL4161450 1 -
CHEMBL4169377 1 -
CHEMBL4161702 1 -
CHEMBL4165128 1 -
CHEMBL4168549 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4175741 EC50 = 63000.0 nM 4.20
CHEMBL4172332 EC50 = 74000.0 nM 4.13
CHEMBL275097 TETRAHYDROBERBERINE EC50 = 93000.0 nM 4.03
CHEMBL4161450 EC50 = 683000.0 nM -
CHEMBL4169377 EC50 = 407000.0 nM -
CHEMBL4161702 EC50 = 119000.0 nM -
CHEMBL4165128 EC50 = 196000.0 nM -
CHEMBL4168549 EC50 = 140000.0 nM -