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Assay Detail

CHEMBL4158378

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at HIF-2alpha in human 786-O cells assessed as reduction in VEGFA concentration after 24 hrs by ELISA
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Endothelial PAS domain-containing protein 1 (CHEMBL1744522)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and Activity of Specific Hypoxia-Inducible Factor-2α (HIF-2α) Inhibitors for the Treatment of Clear Cell Renal Cell Carcinoma: Discovery of Clinical Candidate ( S)-3-((2,2-Difluoro-1-hydroxy-7-(methylsulfonyl)-2,3-dihydro-1 H-inden-4-yl)oxy)-5-fluorobenzonitrile (PT2385).
Wehn PM, Rizzi JP, Dixon DD, Grina JA, Schlachter ST, Wang B, Xu R, Yang H, Du X, Han G, Wang K, Cao Z, Cheng T, Czerwinski RM, Goggin BS, Huang H, Halfmann MM, Maddie MA, Morton EL, Olive SR, Tan H, Xie S, Wong T, Josey JA, Wallace EM.
J Med Chem (2018) 61 : 9691 -9721
PubMed 30289716 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 5 7.63 7.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4165848 1 7.85
CHEMBL4175320 1 7.80
CHEMBL4162398 1 7.70
CHEMBL4167415 1 7.43
CHEMBL4173075 1 7.39

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4165848 EC50 = 14.0 nM 7.85
CHEMBL4175320 EC50 = 16.0 nM 7.80
CHEMBL4162398 EC50 = 20.0 nM 7.70
CHEMBL4167415 EC50 = 37.0 nM 7.43
CHEMBL4173075 EC50 = 41.0 nM 7.39