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Assay Detail

CHEMBL4158682

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Modulation of TRPC1/TRPC3/STIM1/Orai1 in HEK cells assessed as induction of store-operated calcium entry by measuring residual activity preincubated for 30 mins followed by tBhQ-mediated Ca2+ depletion and Ca2+ re-addition to extracellular solution measured for 600 secs by Fluo-4 AM-based fluorometric assay relative to control
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Intermediate

Publication

Pyrtriazoles, a Novel Class of Store-Operated Calcium Entry Modulators: Discovery, Biological Profiling, and in Vivo Proof-of-Concept Efficacy in Acute Pancreatitis.
Riva B, Griglio A, Serafini M, Cordero-Sanchez C, Aprile S, Di Paola R, Gugliandolo E, Alansary D, Biocotino I, Lim D, Grosa G, Galli U, Niemeyer B, Sorba G, Canonico PL, Cuzzocrea S, Genazzani AA, Pirali T.
J Med Chem (2018) 61 : 9756 -9783
PubMed 30347159 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 5.98 6.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL101896 1 6.52
CHEMBL4177187 1 6.30
CHEMBL4162723 1 6.22
CHEMBL4162062 1 5.51
CHEMBL4172741 1 5.36
CHEMBL4173834 1 -
CHEMBL4165917 1 -
CHEMBL4160911 1 -
CHEMBL4176117 1 -
CHEMBL4164770 1 -
CHEMBL4164404 1 -
CHEMBL4165920 1 -
CHEMBL4173839 1 -
CHEMBL4169352 1 -
CHEMBL4177191 1 -
CHEMBL4159089 1 -
CHEMBL4166992 1 -
CHEMBL4162479 1 -
CHEMBL4170397 1 -
CHEMBL4161005 1 -
CHEMBL4168931 1 -
CHEMBL4170347 1 -
CHEMBL4159717 1 -
CHEMBL4166944 1 -
CHEMBL4174834 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL101896 IC50 = 300.0 nM 6.52
CHEMBL4177187 IC50 = 500.0 nM 6.30
CHEMBL4162723 IC50 = 600.0 nM 6.22
CHEMBL4162062 IC50 = 3100.0 nM 5.51
CHEMBL4172741 IC50 = 4400.0 nM 5.36
CHEMBL4173834 IC50 - -
CHEMBL4165917 IC50 - -
CHEMBL4160911 IC50 - -
CHEMBL4176117 IC50 - -
CHEMBL4164770 IC50 - -
CHEMBL4164404 IC50 - -
CHEMBL4165920 IC50 - -
CHEMBL4173839 IC50 - -
CHEMBL4169352 IC50 - -
CHEMBL4177191 IC50 - -
CHEMBL4159089 IC50 - -
CHEMBL4166992 IC50 - -
CHEMBL4162479 IC50 - -
CHEMBL4170397 IC50 - -
CHEMBL4161005 IC50 - -
CHEMBL4168931 IC50 - -
CHEMBL4170347 IC50 - -
CHEMBL4159717 IC50 - -
CHEMBL4166944 IC50 - -
CHEMBL4174834 IC50 - -