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Assay Detail

CHEMBL4178741

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Functional
Antagonist activity at human GPR4 expressed in human HeLa cells assessed as inhibition of IBMX-induced cAMP accumulation after 15 mins by HTRF assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

G-protein coupled receptor 4 (CHEMBL3638324)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of Selective, Orally Active GPR4 Antagonists with Modulatory Effects on Nociception, Inflammation, and Angiogenesis.
Velcicky J, Miltz W, Oberhauser B, Orain D, Vaupel A, Weigand K, Dawson King J, Littlewood-Evans A, Nash M, Feifel R, Loetscher P.
J Med Chem (2017) 60 : 3672 -3683
PubMed 28445047 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 7.27 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3639746 1 7.70
CHEMBL4217603 1 7.68
CHEMBL4208267 1 7.62
CHEMBL4210921 1 7.57
CHEMBL4209248 1 7.37
CHEMBL4213196 1 7.30
CHEMBL4212656 1 7.28
CHEMBL4211343 1 7.20
CHEMBL3675743 1 7.16
CHEMBL4207309 1 7.14
CHEMBL4205308 1 7.13
CHEMBL3675746 1 6.94
CHEMBL4204864 1 6.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3639746 IC50 = 20.0 nM 7.70
CHEMBL4217603 IC50 = 21.0 nM 7.68
CHEMBL4208267 IC50 = 24.0 nM 7.62
CHEMBL4210921 IC50 = 27.0 nM 7.57
CHEMBL4209248 IC50 = 43.0 nM 7.37
CHEMBL4213196 IC50 = 50.0 nM 7.30
CHEMBL4212656 IC50 = 53.0 nM 7.28
CHEMBL4211343 IC50 = 63.0 nM 7.20
CHEMBL3675743 IC50 = 70.0 nM 7.16
CHEMBL4207309 IC50 = 73.0 nM 7.14
CHEMBL4205308 IC50 = 74.0 nM 7.13
CHEMBL3675746 IC50 = 114.0 nM 6.94
CHEMBL4204864 IC50 = 368.0 nM 6.43