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Assay Detail

CHEMBL4178742

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]dofetilide from recombinant human ERG expressed in HEK293 cell membranes after 90 mins by liquid scintillation counting method
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Development of Selective, Orally Active GPR4 Antagonists with Modulatory Effects on Nociception, Inflammation, and Angiogenesis.
Velcicky J, Miltz W, Oberhauser B, Orain D, Vaupel A, Weigand K, Dawson King J, Littlewood-Evans A, Nash M, Feifel R, Loetscher P.
J Med Chem (2017) 60 : 3672 -3683
PubMed 28445047 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 5.18 6.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3675746 1 6.00
CHEMBL4209248 1 5.68
CHEMBL4205308 1 5.64
CHEMBL3639746 1 5.35
CHEMBL4208267 1 5.28
CHEMBL4211343 1 5.24
CHEMBL3675743 1 4.72
CHEMBL4212656 1 4.64
CHEMBL4213196 1 4.62
CHEMBL4207309 1 4.60
CHEMBL4204864 1 -
CHEMBL4210921 1 -
CHEMBL4217603 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3675746 IC50 = 1000.0 nM 6.00
CHEMBL4209248 IC50 = 2100.0 nM 5.68
CHEMBL4205308 IC50 = 2300.0 nM 5.64
CHEMBL3639746 IC50 = 4500.0 nM 5.35
CHEMBL4208267 IC50 = 5300.0 nM 5.28
CHEMBL4211343 IC50 = 5700.0 nM 5.24
CHEMBL3675743 IC50 = 19000.0 nM 4.72
CHEMBL4212656 IC50 = 23000.0 nM 4.64
CHEMBL4213196 IC50 = 24000.0 nM 4.62
CHEMBL4207309 IC50 = 25000.0 nM 4.60
CHEMBL4204864 IC50 > 30000.0 nM -
CHEMBL4210921 IC50 > 30000.0 nM -
CHEMBL4217603 IC50 > 30000.0 nM -