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Assay Detail

CHEMBL4178989

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human TRPV1 expressed in CHO cells assessed as inhibition of capsaicin-induced intracellular calcium level preincubated for 6 mins followed by capsaicin addition by Fluo-4 dye based FLIPR assay
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Pyrazole C-region analogues of 2-(3-fluoro-4-methylsulfonylaminophenyl)propanamides as potent TRPV1 antagonists.
Lee S, Kim C, Ann J, Thorat SA, Kim E, Park J, Choi S, Blumberg PM, Frank-Foltyn R, Bahrenberg G, Stockhausen H, Christoph T, Lee J.
Bioorg Med Chem Lett (2017) 27 : 4383 -4388
PubMed 28838698 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 25 8.47 10.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3689046 1 10.00
CHEMBL3689037 1 10.00
CHEMBL4215829 1 10.00
CHEMBL3689043 1 9.52
CHEMBL3689044 1 9.52
CHEMBL3689036 1 9.52
CHEMBL3689041 1 9.40
CHEMBL3689038 1 9.30
CHEMBL3689034 1 8.43
CHEMBL4205905 1 8.29
CHEMBL4217897 1 8.10
CHEMBL3684333 1 8.10
CHEMBL3684332 1 7.67
CHEMBL3689045 1 7.58
CHEMBL3684330 1 7.56
CHEMBL3689042 1 7.50
CHEMBL4203430 1 7.30
CHEMBL3689033 1 7.12
CHEMBL4209620 1 6.09
CHEMBL4202633 1 -
CHEMBL4205551 1 -
CHEMBL4215629 1 -
CHEMBL4205870 1 -
CHEMBL3689040 1 -
CHEMBL4215102 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3689046 Ki = 0.1 nM 10.00
CHEMBL3689037 Ki = 0.1 nM 10.00
CHEMBL4215829 Ki = 0.1 nM 10.00
CHEMBL3689043 Ki = 0.3 nM 9.52
CHEMBL3689044 Ki = 0.3 nM 9.52
CHEMBL3689036 Ki = 0.3 nM 9.52
CHEMBL3689041 Ki = 0.4 nM 9.40
CHEMBL3689038 Ki = 0.5 nM 9.30
CHEMBL3689034 Ki = 3.7 nM 8.43
CHEMBL4205905 Ki = 5.1 nM 8.29
CHEMBL4217897 Ki = 8.0 nM 8.10
CHEMBL3684333 Ki = 8.0 nM 8.10
CHEMBL3684332 Ki = 21.6 nM 7.67
CHEMBL3689045 Ki = 26.5 nM 7.58
CHEMBL3684330 Ki = 27.7 nM 7.56
CHEMBL3689042 Ki = 31.7 nM 7.50
CHEMBL4203430 Ki = 50.2 nM 7.30
CHEMBL3689033 Ki = 75.2 nM 7.12
CHEMBL4209620 Ki = 819.0 nM 6.09
CHEMBL4202633 Ki - -
CHEMBL4205551 Ki - -
CHEMBL4215629 Ki - -
CHEMBL4205870 Ki - -
CHEMBL3689040 Ki - -
CHEMBL4215102 Ki - -