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Assay Detail

CHEMBL4180673

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of 1,8-ANS from human N-terminal His-tagged RORgammat LBD (850 to 1635 residues) expressed in Escherichia coli by thermofluor assay
33
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Nuclear receptor ROR-gamma (CHEMBL1741186)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

6-Substituted quinolines as RORγt inverse agonists.
Barbay JK, Cummings MD, Abad M, Castro G, Kreutter KD, Kummer DA, Maharoof U, Milligan C, Nishimura R, Pierce J, Schalk-Hihi C, Spurlino J, Tanis VM, Urbanski M, Venkatesan H, Wang A, Woods C, Wolin R, Xue X, Edwards JP, Fourie AM, Leonard K.
Bioorg Med Chem Lett (2017) 27 : 5277 -5283
PubMed 29079472 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 33 7.50 9.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4216372 1 9.57
CHEMBL4204175 2 9.42
CHEMBL4205065 1 9.30
CHEMBL4217859 1 9.22
CHEMBL4209944 1 8.96
CHEMBL3964680 2 8.44
CHEMBL3952077 1 8.30
CHEMBL3968603 2 8.30
CHEMBL4209998 1 8.28
CHEMBL4218386 1 8.19
CHEMBL3957588 2 8.04
CHEMBL4094197 1 7.85
CHEMBL3972220 2 7.70
CHEMBL4213458 1 7.51
CHEMBL4071285 3 7.50
CHEMBL4214388 1 7.42
CHEMBL4209983 2 7.16
CHEMBL4205629 1 7.12
CHEMBL4218239 1 6.96
CHEMBL4207122 1 6.72
CHEMBL4206508 1 6.55
CHEMBL4211999 1 6.35
CHEMBL4211066 1 6.02
CHEMBL4208253 1 5.82
CHEMBL4210499 1 4.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4216372 Kd = 0.27 nM 9.57
CHEMBL4204175 Kd = 0.38 nM 9.42
CHEMBL4205065 Kd = 0.5 nM 9.30
CHEMBL4217859 Kd = 0.6 nM 9.22
CHEMBL4209944 Kd = 1.1 nM 8.96
CHEMBL4204175 Kd = 1.2 nM 8.92
CHEMBL3964680 Kd = 3.6 nM 8.44
CHEMBL3952077 Kd = 5.0 nM 8.30
CHEMBL3968603 Kd = 5.0 nM 8.30
CHEMBL4209998 Kd = 5.3 nM 8.28
CHEMBL4218386 Kd = 6.4 nM 8.19
CHEMBL3957588 Kd = 9.2 nM 8.04
CHEMBL4094197 Kd = 14.0 nM 7.85
CHEMBL3972220 Kd = 20.0 nM 7.70
CHEMBL4213458 Kd = 31.0 nM 7.51
CHEMBL4071285 Kd = 32.0 nM 7.50
CHEMBL3972220 Kd = 33.0 nM 7.48
CHEMBL4214388 Kd = 38.0 nM 7.42
CHEMBL3968603 Kd = 70.0 nM 7.16
CHEMBL4209983 Kd = 69.0 nM 7.16
CHEMBL4205629 Kd = 76.0 nM 7.12
CHEMBL3964680 Kd = 77.0 nM 7.11
CHEMBL4209983 Kd = 91.0 nM 7.04
CHEMBL4218239 Kd = 110.0 nM 6.96
CHEMBL4071285 Kd = 160.0 nM 6.80
CHEMBL4207122 Kd = 190.0 nM 6.72
CHEMBL4206508 Kd = 280.0 nM 6.55
CHEMBL4211999 Kd = 450.0 nM 6.35
CHEMBL3957588 Kd = 520.0 nM 6.28
CHEMBL4211066 Kd = 960.0 nM 6.02
CHEMBL4208253 Kd = 1500.0 nM 5.82
CHEMBL4071285 Kd = 5700.0 nM 5.24
CHEMBL4210499 Kd = 20000.0 nM 4.70