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Assay Detail

CHEMBL4180674

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inverse agonist activity at GAL4 DBD-fused wild type human RORgammat LBD (850 to 1635 residues) expressed in HEK293T cells assessed as inhibition of transcriptional activity after 24 hrs by dual-glo luciferase reporter gene assay
33
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Nuclear receptor ROR-gamma (CHEMBL1741186)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

6-Substituted quinolines as RORγt inverse agonists.
Barbay JK, Cummings MD, Abad M, Castro G, Kreutter KD, Kummer DA, Maharoof U, Milligan C, Nishimura R, Pierce J, Schalk-Hihi C, Spurlino J, Tanis VM, Urbanski M, Venkatesan H, Wang A, Woods C, Wolin R, Xue X, Edwards JP, Fourie AM, Leonard K.
Bioorg Med Chem Lett (2017) 27 : 5277 -5283
PubMed 29079472 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 33 6.92 8.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4204175 2 8.28
CHEMBL4205065 1 8.26
CHEMBL3952077 1 8.18
CHEMBL4217859 1 7.89
CHEMBL4209998 1 7.68
CHEMBL3964680 2 7.62
CHEMBL3972220 2 7.34
CHEMBL4094197 1 7.30
CHEMBL3957588 2 7.14
CHEMBL4218386 1 7.09
CHEMBL3968603 2 7.05
CHEMBL4209944 1 7.00
CHEMBL4205629 1 6.89
CHEMBL4214388 1 6.85
CHEMBL4213458 1 6.75
CHEMBL4216372 1 6.70
CHEMBL4218239 1 6.68
CHEMBL4071285 3 6.66
CHEMBL4209983 2 6.57
CHEMBL4207122 1 6.22
CHEMBL4206508 1 6.20
CHEMBL4211999 1 6.09
CHEMBL4211066 1 6.00
CHEMBL4208253 1 5.68
CHEMBL4210499 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4204175 IC50 = 5.3 nM 8.28
CHEMBL4205065 IC50 = 5.5 nM 8.26
CHEMBL3952077 IC50 = 6.6 nM 8.18
CHEMBL4217859 IC50 = 13.0 nM 7.89
CHEMBL4204175 IC50 = 13.0 nM 7.89
CHEMBL4209998 IC50 = 21.0 nM 7.68
CHEMBL3964680 IC50 = 24.0 nM 7.62
CHEMBL3972220 IC50 = 46.0 nM 7.34
CHEMBL4094197 IC50 = 50.0 nM 7.30
CHEMBL3972220 IC50 = 59.0 nM 7.23
CHEMBL3957588 IC50 = 73.0 nM 7.14
CHEMBL4218386 IC50 = 82.0 nM 7.09
CHEMBL3968603 IC50 = 89.0 nM 7.05
CHEMBL4209944 IC50 = 100.0 nM 7.00
CHEMBL4205629 IC50 = 130.0 nM 6.89
CHEMBL3964680 IC50 = 140.0 nM 6.85
CHEMBL4214388 IC50 = 140.0 nM 6.85
CHEMBL3968603 IC50 = 170.0 nM 6.77
CHEMBL4213458 IC50 = 180.0 nM 6.75
CHEMBL4216372 IC50 = 200.0 nM 6.70
CHEMBL4218239 IC50 = 210.0 nM 6.68
CHEMBL4071285 IC50 = 220.0 nM 6.66
CHEMBL4071285 IC50 = 260.0 nM 6.58
CHEMBL4209983 IC50 = 270.0 nM 6.57
CHEMBL4209983 IC50 = 300.0 nM 6.52
CHEMBL4207122 IC50 = 600.0 nM 6.22
CHEMBL4206508 IC50 = 630.0 nM 6.20
CHEMBL3957588 IC50 = 670.0 nM 6.17
CHEMBL4211999 IC50 = 820.0 nM 6.09
CHEMBL4211066 IC50 = 1000.0 nM 6.00
CHEMBL4208253 IC50 = 2100.0 nM 5.68
CHEMBL4071285 IC50 = 5500.0 nM 5.26
CHEMBL4210499 IC50 > 6000.0 nM -