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Assay Detail

CHEMBL4180810

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of CDK2/cyclin E (unknown origin) expressed in baculovirus infected insect Sf9 cells using histone H1 as substrate in presence of [gamma-33P]ATP
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 7 — Homologous single protein target
Curated By Intermediate

Target

Cyclin-dependent kinase 2/cyclin E1 (CHEMBL1907605)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Trisubstituted purine inhibitors of PDGFRα and their antileukemic activity in the human eosinophilic cell line EOL-1.
Malínková V, Řezníčková E, Jorda R, Gucký T, Kryštof V.
Bioorg Med Chem (2017) 25 : 6523 -6535
PubMed 29089259 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 6.28 7.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL367625 1 7.77
CHEMBL4209971 1 6.93
CHEMBL4207053 1 6.88
CHEMBL4202703 1 6.87
CHEMBL4218867 1 6.68
CHEMBL4207048 1 6.64
CHEMBL4210919 1 6.56
CHEMBL4205769 1 6.51
CHEMBL4218356 1 6.50
CHEMBL4215315 1 6.36
CHEMBL4214362 1 6.36
CHEMBL4214422 1 6.31
CHEMBL4206527 1 6.31
CHEMBL4218779 1 6.30
CHEMBL4205604 1 6.29
CHEMBL4210474 1 6.20
CHEMBL4209694 1 6.17
CHEMBL4211413 1 6.01
CHEMBL4216607 1 6.01
CHEMBL4210897 1 5.84
CHEMBL4218942 1 5.55
CHEMBL4214781 1 5.26
CHEMBL4207999 1 5.22
CHEMBL4203120 1 5.14
CHEMBL554 LAPATINIB 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL367625 IC50 = 17.0 nM 7.77
CHEMBL4209971 IC50 = 117.0 nM 6.93
CHEMBL4207053 IC50 = 132.0 nM 6.88
CHEMBL4202703 IC50 = 134.0 nM 6.87
CHEMBL4218867 IC50 = 207.0 nM 6.68
CHEMBL4207048 IC50 = 227.0 nM 6.64
CHEMBL4210919 IC50 = 275.0 nM 6.56
CHEMBL4205769 IC50 = 306.0 nM 6.51
CHEMBL4218356 IC50 = 319.0 nM 6.50
CHEMBL4215315 IC50 = 442.0 nM 6.36
CHEMBL4214362 IC50 = 433.0 nM 6.36
CHEMBL4214422 IC50 = 490.0 nM 6.31
CHEMBL4206527 IC50 = 485.0 nM 6.31
CHEMBL4218779 IC50 = 500.0 nM 6.30
CHEMBL4205604 IC50 = 513.0 nM 6.29
CHEMBL4210474 IC50 = 627.0 nM 6.20
CHEMBL4209694 IC50 = 680.0 nM 6.17
CHEMBL4211413 IC50 = 980.0 nM 6.01
CHEMBL4216607 IC50 = 965.0 nM 6.01
CHEMBL4210897 IC50 = 1441.0 nM 5.84
CHEMBL4218942 IC50 = 2830.0 nM 5.55
CHEMBL4214781 IC50 = 5504.0 nM 5.26
CHEMBL4207999 IC50 = 5967.0 nM 5.22
CHEMBL4203120 IC50 = 7170.0 nM 5.14
CHEMBL554 LAPATINIB IC50 > 20000.0 nM -