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Assay Detail

CHEMBL4183135

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Displacement of [3H]-PF-6475886 from recombinant human full length Myc-DDK-tagged BACE2 expressed in HEK293 cell membranes after 30 mins by parallel scintillation proximity assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-secretase 2 (CHEMBL2525)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and Synthesis of Clinical Candidate PF-06751979: A Potent, Brain Penetrant, β-Site Amyloid Precursor Protein Cleaving Enzyme 1 (BACE1) Inhibitor Lacking Hypopigmentation.
O'Neill BT, Beck EM, Butler CR, Nolan CE, Gonzales C, Zhang L, Doran SD, Lapham K, Buzon LM, Dutra JK, Barreiro G, Hou X, Martinez-Alsina LA, Rogers BN, Villalobos A, Murray JC, Ogilvie K, LaChapelle EA, Chang C, Lanyon LF, Steppan CM, Robshaw A, Hales K, Boucher GG, Pandher K, Houle C, Ambroise CW, Karanian D, Riddell D, Bales KR, Brodney MA.
J Med Chem (2018) 61 : 4476 -4504
PubMed 29613789 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 7.58 8.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3672916 1 8.48
CHEMBL4205912 1 7.75
CHEMBL3967984 1 7.70
CHEMBL3972449 1 7.24
CHEMBL3952064 1 6.71

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3672916 IC50 = 3.3 nM 8.48
CHEMBL4205912 IC50 = 17.8 nM 7.75
CHEMBL3967984 IC50 = 20.0 nM 7.70
CHEMBL3972449 IC50 = 57.0 nM 7.24
CHEMBL3952064 IC50 = 194.0 nM 6.71